IDENTIFICATION OF BACTERIUM Escherichia coli IN PELAGIC AND DEMERSAL FISH BY CULTURE AND POLYMERASE CHAIN REACTION (PCR) METHODS

Iftachul Farida; Ni Putu Kumala Dewi

doi:10.15578/aj.v6i2.13717

IDENTIFICATION OF BACTERIUM Escherichia coli IN PELAGIC AND DEMERSAL FISH BY CULTURE AND POLYMERASE CHAIN REACTION (PCR) METHODS

Iftachul Farida, Ni Putu Kumala Dewi

Abstract

Fish is a commodity that easily and quickly decomposes (high perishable food), therefore, since being caught from the sea, it requires fast, clean, careful and cool handling so that when it is distributed or marketed to fish consumers in fresh condition and maintained quality. The large number of pelagic fish and demersal fish exported to foreign countries requires that the exported fish be tested for its content, especially for microbial contamination, one of which is Escherichia coli contamination. The purpose of this study was to identify Escherichia coli bacteria in Pelagic and Demersal fish using culture and Polymerase Chain Reaction (PCR) methods. Identification of E. coli bacteria used two methods, namely the culture method and PCR using the lacZ gene primer. The results showed that the use of culture and PCR testing methods in identifying E. coli bacteria in demersal and pelagic fish showed the same results, namely 7 (47%) positive samples containing E. coli contamination with the same sample code and exceeding the limit for bacterial E contamination. coli according to SNI 2729:2013 regarding fresh fish, namely <3 APM/g.

Keywords

contamination, lacZ gene, microbes

Full Text:

PDF

References

Bambang, A.G., Fatimawali., Novel S.K., (2014) Analisis cemaran bakteri coliform dan identifikasi Escherichia coli pada air isi ulang dari depot di kota Manado. Pharmacon Jurnla Ilmiah Farmasi. Vol. 3 No. 3. ISSN 2302-2493.

[BSN] Badan Standarisasi Nasional. (2013). SNI 2729:2013 tentang Ikan Segar. Diterbitkan di Jakarta.

[BSN] Badan Standardisasi Nasional. (2006). SNI Ikan Segar – Bagian 2: Persyaratan Bahan Baku (01-2729.2-2006). Jakarta. Badan Standardisasi Nasional.

Bakri, Z., M. Hatta., Muh. N.M. (2015). Deteksi keberadaan bakteri Escherichia coli o157:H7 pada feses penderita diare dengan metode kultur dan PCR. JST Kesehatan, April 2015, Vol. 5 No. 2: 184 – 192.

Buktiwetan P, Surjawidjaja J, Salim O, Aidilifit M, Lesmana M. (2001). Diare bakterial: etiologi dan kepekaan Antibiotika di dua Pusat Kesehatan Masyarakat di Jakarta. Jkedokter Trisakti 2001: 20:57-65.

Brooks, GF., Jawetz, Melnick & Adelberg’s. Medical Microbiology 25th edition. (2010) New York: McGraw-Hill Companies. Chapter 15.

Christanti, S.D., Azhar, M.H. (2019). Identifikasi bakteri Escherichia coli dan Salmonella sp. pada produk beku perikanan di Balai Karantina Ikan, Pengendalian Mutu, dan Keamanan Hasil Perikanan Surabaya II, Jawa Timur. Journal of Aquaculture Science. Oktober 2019 Vol 4 (2): 62-72.

Fialho, O. B., Souza, E. M. De, Dallagassa, C. D. B., Pedrosa, D. O., Klassen, G., Irino, K., Paludo, K. S., Assis, D., Surek, M., Ara, E., Souza, M. De, Farah, S., & Fadel-picheth, C. M. T. (2013). Detection of Diarrheagenic Escherichia coli Using a Two-System Multiplex-PCR Protocol. Journal of Clinical Laboratory Analysis, 161(January), 155–161. https://doi. org/10.1002/jcla.21578.

Hermawan, (2018). Uji Bakteri Fecal Coliform pada Cincau Hitam yang berada di Pasar Segiri Samarinda. [skripsi]. Universitas Muhammadiyah Kalimantan Timur.

Isfahani, B. N., Fazeli, H., Babaie, Z., Poursina, F., Moghim, S., & Rouzbahani, M. (2017). Evaluation of Polymerase Chain Reaction for Detecting Coliform Bacteria in Drinking Water Sources. Advanced Biomedical Research, 1–3. https://doi. org/10.4103/2277-9175.216783.

Metusalach, Kasmiati, Fahrul, Jaya I. (2014). Pengaruh Transportasi Terhadap Mutu Dan Harga Ikan Dari Pelabuhan Perikanan Pantai Lempasing Ke Daerah Konsumen. Jurnal Albacore, Volume 2, No 2, Juni 2018, Hal 209-219.

Molina, F., López-acedo, E., Tabla, R., Roa, I., Gómez, A., & Rebollo, J. E. (2015). Improved detection of Escherichia coli and coliform bacteria by multiplex PCR. BMC Biotechnology, 1–9. https://doi. org/10.1186/s12896-015-0168-2.

Nur Hasanah., Putu E.S., Imanuddin R., Ni Nyoman E., Wahyu A.N., Hidayati K., Ni Putu S.S.A., Ida Ayu M.M.D., (2022). Deteksi dini kontaminasi Vibrio parahaemolyticus dan Escherichia coli pada produk perikanan dengan Multiplez Polymerase Chain Reaction. Jurnal Sain Veteriner, Vol. 40 No. 2 Agustus 2022, Hal. 171-182.

Zhang, W., Wang, C., Huang, C. Y., Yu, Q., Liu, H. C., Zhang, C. W., & Pei, X. F. (2012). Construction and Secretory Expression of β-Galactosidase Gene from Lactobacillus Bulgaricus in Lactococcus Lactis. Biomedical and Environmental Sciences, 25(2), 203–209. https://doi. org/10.3967/0895-3988.2012.02.012.

DOI: http://dx.doi.org/10.15578/aj.v6i2.13717