IDENTIFIKASI JENIS DAN HUBUNGAN KELIMPAHAN LARVA IKAN DENGAN KUALITAS AIR DI DANAU RANAU, SUMATERA SELATAN

Tuah Nanda Merlia Wulandari, Herlan Herlan, Arif Wibowo, Sevi Sawetri

Abstract


Identifikasi larva ikan seringkali sulit dilakukan. Belum tersedianya kunci identifikasi untuk spesies larva ikan di perairan umum daratan sehingga belum adanya pedoman untuk dapat mengidentifikasi spesies larva ikan secara morfologi maupun meristik. Penelitian ini bertujuan untuk mengidentifikasi spesies larva ikan melalui sekuen DNA sehingga menghasilkan data spesies yang lebih pasti dan hubungan kelimpahan larva ikan dengan parameter kualitas perairan di Danau Ranau, Sumatera Selatan. Lokasi pengambilan sampel identifikasi jenis dan kualitas perairan di enam stasiun di Perairan Danau Ranau, Sumatera Selatan. Hasil identifikasi dengan menggunakan sekuen DNA ditemukan sepuluh jenis larva ikan. Kesepuluh jenis larva ikan tersebut antara lain Rasbora argyrotaenia, Puntius tetrazona, Oreochromis mossambicus, Oreochromis niloticus, Gambusia affinis, Poecilia reticulata, Mystacoleucus marginatus, Channa striata, Trichopsis vittata, and Trichogaster trichopterus. Sekuen DNA kemudian disejajarkan dengan sekuen referen dari perpustakaan data bank gen untuk mengakar pohon dengan menggunakan program BLAST dari NCBI untuk menemukan identitas yang paling dekat keterkaitannya dengan sampel larva-larva ikan yang dianalisis. Hubungan filogeni spesies diantara takson larva-larva ikan di Danau Ranau berdekatan. Kondisi perairan di Danau Ranau tergolong baik untuk biota perairan seperti larva ikan. Kualitas air merupakan parameter yang menentukan kelimpahan larva ikan di Danau Ranau. Larva-larva ikan antar populasi memiliki jarak genetik yang dekat antar satu dengan yang lain. Kelimpahan larva ikan berkorelasi positif dengan turbiditas dan berkorelasi negatif terhadap parameter daya hantar listrik.

Identification of fish larva is often difficult. The unavailability of key identification for fish larva species in inland waters so that there are no guidelines for identifying fish larvae morphologically or meristically. This study aims to identify fish larva species from the Ranau Lake waters, South Sumatra through DNA sequences to produce more definitive species data and relationship of abundance of fish larva and water quality of parameters in Ranau Lake waters, South Sumatera. The sampling location of species identification and water quality was six stations in Ranau Lake waters, South Sumatra. The results of the study found the identification of fish larva species using DNA sequence found ten types of fish larva obtained. The ten types of fish larva include Rasbora argyrotaenia, Puntius tetrazona, Oreochromis mossambicus, Oreochromis niloticus, Gambusia affinis, Poecilia reticulata, Mystacoleucus marginatus, Channa striata, Trichopsis vittata, and Trichogaster trichopterus. The DNA sequence was then aligned with the referent sequence from the gene bank data library to root the tree by using the BLAST program from NCBI to find the identity that was closest to the sample of fish larvae analyzed. The relationship of species phylogeny between the fish larva of fish larva in Ranau Lake is close together. Fish larva between populations have close genetic distance from one another. Water quality in Ranau Lake are good for aquatic biota such as fish larva. Water quality is a parameter that determines the abundance of fish larva in Ranau Lake. The abundance of fish larva was positively correlated with turbidity, whereas negatively correlated to conductivity parameters.


Keywords


Larva ikan; Kualitas perairan; Sekuen DNA; Danau Ranau

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References


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DOI: http://dx.doi.org/10.15578/bawal.11.1.2019.33-44


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