PENGARUH PENAMBAHAN KULTUR STARTER DAN METABOLIT Lactobacillus casei TERHADAP MUTU MIKROBIOLOGI SOSIS FERMENTASI IKAN PATIN (Pangasius sp.)

Muhammad Alfid Kurnianto; Hadi Munarko

doi:10.15578/jkpt.v5i1.10970

PENGARUH PENAMBAHAN KULTUR STARTER DAN METABOLIT Lactobacillus casei TERHADAP MUTU MIKROBIOLOGI SOSIS FERMENTASI IKAN PATIN (Pangasius sp.)

Muhammad Alfid Kurnianto, Hadi Munarko

Abstract

Sosis fermentasi ikan patin merupakan salah satu bentuk diversifikasi produk olahan ikan patin yang dibuat dengan teknologi fermentasi menggunakan bakteri asam laktat (BAL). Penambahan starter BAL pada pengolahan sosis fermentasi biasanya dilakukan untuk menghasilkan produk yang terstandar dan aman. Penelitian ini bertujuan untuk mengevaluasi pengaruh penambahan kultur bakteri dan metabolit hasil produksi Lactobacillus casei secara individu dan campuran terhadap mutu dan keamanan mikrobiologi sosis fermentasi ikan patin. Hasil penelitian menunjukkan bahwa produk sosis fermentasi ikan patin memiliki pH dan aktivitas air (a_w) dengan rentang 4,42 – 4,73 dan 0,967 – 0,978. Pemberian perlakuan secara signifikan mempengaruhi mutu mikrobiologi sosis fermentasi ikan patin. Perlakuan penambahan BAL maupun kombinasi BAL dan metabolit setelah pematangan 28 hari memiliki kandungan angka lempeng total (ALT) dan total Enterobacteriaceae tidak berbeda nyata, serta tidak ditemukan cemaran E. coli dan Salmonella sp. Sementara itu, perlakuan penambahan metabolit memiliki ALT dan BAL terendah, serta ditemukan cemaran E. coli pada produk setelah pematangan 28 hari. Perlakuan kombinasi BAL dan metabolit merupakan perlakuan terbaik karena mampu menjaga produk tetap memenuhi standar keamanan mikrobiologi sosis hingga masa pematangan selesai dengan kandungan BAL tertinggi.

Keywords

Bakteri asam laktat; produk fermentasi ikan; metabolit bakteri; keamanan pangan; mutu mikrobiologis

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DOI: http://dx.doi.org/10.15578/jkpt.v5i1.10970