IDENTIFIKASI SENYAWA POTENSIAL ANTIOKSIDAN PADA MAKROALGA COKELAT Turbinaria ornata DARI PANTAI GUNUNGKIDUL, YOGYAKARTA
Abstract
Makroalga cokelat memiliki kandungan senyawa bioaktif yang bermanfaat dalam berbagai bidang kesehatan. Salah satu jenis makroalga cokelat dengan potensi antioksidan adalah Turbinaria ornata. Penelitian ini bertujuan untuk mengidentifikasi kandungan senyawa potensial antioksidannya. Fraksinasi terhadap ekstrak T. ornata dilakukan untuk memfokuskan jenis senyawa potensial antioksidan. Ekstrak dan fraksi dengan potensi antioksidan terbaik dianalisis kandungan senyawanya dengan gas chromatography-mass spectrometry (GC-MS). Pemantauan dengan plat kromatografi lapis tipis (KLT) juga dilakukan terhadap fraksi dengan potensi antioksidan terbaik. Hasil analisis GC-MS dan KLT mengidentifkasi senyawa hidrokarbon dodecane, asam lemak ester hexadecanoic acid ethyl ester, monoterpenoid dyhidroacnidiolide dan loliolide, senyawa fenol resorcinol serta flavonoid kaempherol sebagai senyawa-senyawa potensial pendukung aktivitas antioksidan makroalga cokelat T. ornata. Study menyimpulkan bahwa senyawa-senyawa tersebut memiliki potensi dalam mendukung aktivitas antioksidan makroalga cokelat T. ornata.
Brown macroalgae contain abundant bioactive compounds and are used in various medical applications. Turbinaria ornata is one of the brown macroalgae species suspected to have promising antioxidant potential. This recent study was conducted to identify the potential antioxidant compounds in T. ornata. Fractionation of T. ornata extract was done to differentiate the types of potential antioxidant compounds. Extracts and fractions with the best antioxidant potential were analyzed using gas chromatography-mass spectrometry (GC-MS). The fractions with the best antioxidant potential were monitored using the thin layer chromatography (TLC) plate. The analyses have determined the presence of bioactive compounds such as dodecane, hexadecanoic acid ethyl ester, monoterpenoids dyhidroacnidiolide and loliolide, resorcinol and kaempherol in the tested samples of brown macroalga T. ornata. These compounds have measurable effects on the antioxidant activity of the brown macroalgae. This study concluded that the identified bioactive compounds are deemed as potential compounds supporting the antioxidant activity of the brown macroalgae T. ornata.
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DOI: http://dx.doi.org/10.15578/jra.17.3.2022.155-167
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