SUPLEMENTASI L-KARNITIN DAN KAYU MANIS PADA PAKAN TERHADAP PENURUNAN LEMAK DAN TEKSTUR FILET IKAN PATIN Pangasianodon hypophthalmus PADA FASE PEMBESARAN

Imam Tri Wahyudi, Dedi Jusadi, Mia Setiawati, Julie Ekasari, Muhammad Agus Suprayudi

Abstract


upaya untuk mengurangi kadar lemak tersebut. Tujuan penelitian ini adalah mengevaluasi suplementasi L-karnitin dan tepung kayu manis terhadap kadar lemak dan tekstur daging ikan patin pada fase pembesaran. Ikan patin berukuran 125,4 ± 7,85 g dan panjang 24,71±0,68 cm dipelihara selama 60 hari dalam wadah hapa berukuran 2 x 1 x 1 m3. Ikan diberi pakan tiga kali sehari ad satiation dengan perlakuan, sebagai berikut: kontrol (K), penambahan L-karnitin 1 g kg-1 (LK 1), L-karnitin 2 g kg-1 (LK 2), kayu manis 5 g kg-1 (KM 5), dan kayu manis 10 g kg-1 (KM 10). Sampel diambil pada awal, pertengahan, dan akhir pemeliharaan untuk pengukuran kadar lemak daging. Parameter yang diamati yaitu lemak daging dan tekstur daging. Hasil menunjukkan bahwa pemberian KM 10 menghasilkan kadar lemak yang lebih rendah dari perlakuan lainnya setelah 30 hari dan penurunan yang lebih besar yaitu berkisar 51,06% dan 42,55% pada perlakuan LK 2 dan KM 10 pada hari ke-60. Nilai indeks hepatosomatik juga menurun yang diikuti oleh penurunan kadar lemak hati. Nilai kekerasan daging menunjukkan peningkatan kualitas yang terlihat dari nilai yang semakin rendah. Pemberian tepung kayu manis 10 g kg-1 pada fase pembesaran menunjukkan hasil terbaik pada pemberian selama 60 hari dalam menurunkan lemak daging ikan patin hingga memenuhi standar filet, karena adanya proses lipolisis serta pemanfaatan lemak menjadi energi. Kayu manis ini menjadi sangat potensial untuk dikembangkan sebagai feed additive dalam upaya memperbaiki kualitas daging ikan patin fase pembesaran.

Striped catfish meat has a high-fat content which is undesirable to markets and customers alike and needs to be reduced. This study aims to evaluate the effect of dietary L-carnitine and cinnamon powder on reducing the fat content of striped catfish meat in the grow-out stage. Striped catfish measuring 125.4 ± 7.85 g and 24.71 ± 0.68 cm body length were kept for 60 days in a hapa with size 2 x 1 x 1 m3. Thefish were fed three times a day at satiation with the following treatments: control (K), the addition of L-carnitine 1 g kg-1 (LK 1), L-carnitine 2 g kg-1 (LK 2), cinnamon 5 g kg-1 (KM 5) and cinnamon 10 g kg-1 (KM 10). Sampling was conducted on the initial, middle and final day for meat fat content analysis. The parameters observed were meat fat and meat texture. The results showed that dietary KM 10 significantly reduced meat fat content compared to controls on the 30th and it showed a more significant reduction, namely 51.06% and 42.55%, in the treatment LK 2 and KM 10 after 60 days treatment. The hepatosomatic index value also decreased, followed by decreased liver fat levels. The lower values of meat hardness imply an increase in meat quality. It can be concluded that the application of dietary cinnamon powder at 10 g kg-1 (KM 10) is the best level to reduce the fat content of striped catfish meat in meeting the fillet standards. This cinnamon has excellent potential to be developed as a feed additive to improve the quality of striped catfish meat in the rearing phase.


Keywords


ikan patin; kayu manis; lemak daging; L-karnitin; tekstur; cinnamon; L-carnitine; lipid content; striped catfish; texture

Full Text:

PDF

References


Abd El-Hamid, M.I., Ibrahim, S.M., Eldemery, F., El-Mandrawy, S.A.M., Metwally, A.S., Khalifa, E., Elnahriry, S.S., & Ibrahim, D. (2021). Dietary cinnamaldehyde nanoemulsion boosts growth and transcriptomes of antioxidant and immune related genes to fight Streptococcus agalactiae infection in Nile tilapia (Oreochromis niloticus). Fish & Shellfish Immunology, 113, 96–105. https://doi.org/10.1016/j.fsi.2021.03.021

Akbari, M., Rahimabadi, E.Z., Sorinejad, I., Rad, M.A., Efatpanah, E., & Khanjani, M.H. (2014). Effect of different levels of dietary L-carnitine on growth performance, food Efficiency and body composition of pikeperch (Sander lucioperca) fingerlings. World Journal of Fish and Marine Sciences, 6(3), 7–232. https://doi.org/10.5829/idosi.wjfms.2014.06.03.82416

Ali, S.SR., Ramachandran, M., Chakma, S.K., & Asrar, M. (2017). Proximate composition of commercially important marine fishes and shrimps from the Chennai coast, India. International Journal of Fisheries and Aquatic Studies, 5(5), 113–119.

Al-Noor, S.M., Hossain, M.D., & Islam, M.A. (2012). The study of fillet proximate composition, growth performance and survival rate of striped catfish (Pangasius hypophthalmus) fed with diets containing different amounts of alpha-tocopherol (vitamin-E). Journal of Bio-Science, 20, 67–74. https://doi.org/10.3329/jbs.v20i0.17658

AOAC. (2012). Official Methods of Analysis of AOAC International (19th ed.). AOAC International.

Azaria, S., & Van Rijn, J. (2018). Off-flavor compounds in recirculating aquaculture systems (RAS): Production and removal processes. Aquacultural Engineering, 83, 57–64. https://doi.org/10.1016/j.aquaeng.2018.09.004

Bland, J.M., Bett-Garber, K.L., Li, C.H., Brashear, S.S., Lea, J.M., & Bechtel, P.J. (2018). Comparison of sensory and instrumental methods for the analysis of texture of cooked individually quick frozen and fresh-frozen catfish fillets. Food Science & Nutrition, 6(6), 1692–1705. https://doi.org/10.1002/fsn3.737

BSN. (2016). Ikan Patin Siam (Pangasianodon hypophthalmus, Sauvage 1878)—Bagian 3: Produksi Induk. Badan Standardisasi Nasional.

Chen, Z., Jing, F., Lu, M., Su, C., Tong, R., & Pan, L. (2022). Effects of dietary trans-cinnamaldehyde on growth performance, lipid metabolism, immune response and intestinal microbiota of Litopenaeus vannamei. Fish & Shellfish Immunology, 131, 908–917. https://doi.org/10.1016/j.fsi.2022.11.008

Cheng, J.-H., Sun, D.-W., Han, Z., & Zeng, X.-A. (2014). Texture and structure measurements and analyses for evaluation of fish and fillet freshness quality: A review: fish and fillet freshness quality: a review. Comprehensive Reviews in Food Science and Food Safety, 13(1), 52–61. https://doi.org/10.1111/1541-4337.12043

FAO. (2022). The State of World Fisheries and Aquaculture 2022. FAO. https://doi.org/10.4060/cc0461en

Global Aquaculture Alliance. (2020). The Advocate Global Aquaculture Alliance. https://www.aquaculturealliance.org/advocate/goal-2020-finfish-production-survey/.

Gopan, A., Ande, M.P., Varghese, T., Sahu, N.P., Lalappan, S., Srivastava, P.P., & Jain, K.K. (2018). Dietary carotenoid supplementation improves fillet appearance, antioxidant status and immuneresponses in striped catfish (Pangasianodon hypophthalmus) neverthless the growth performance. Turkish Journal of Fisheries and Aquatic Sciences, 18(11). https://doi.org/10.4194/1303-2712-v18_11_07

Gu, Y., Han, J., Wang, W., Zhan, Y., Wang, H., Hua, W., Liu, Y., Guo, Y., Xue, Z., & Wang, W. (2022). Dietary cinnamaldehyde enhances growth performance, digestion, immunity, and lipid metabolism in juvenile fat greenling (Hexagrammos otakii). Aquaculture Nutrition, 2022, 1–12. https://doi.org/10.1155/2022/2132754

Guimarães, C.F.M., Mársico, E.T., Monteiro, M.L.G., Lemos, M., Mano, S.B., & Conte Junior, C.A. (2016). The chemical quality of frozen Vietnamese Pangasius hypophthalmus fillets. Food Science & Nutrition, 4(3), 398–408. https://doi.org/10.1002/fsn3.302

Harimana, Y., Tang, X., Xu, P., Xu, G., Karangwa, E., Zhang, K., Sun, Y., Li, Y., Ma, S., Uriho, A., & Tuyishimire, M. A. (2019). Effect of long-term moderate exercise on muscle cellularity and texture, antioxidant activities, tissue composition, freshness indicators and flavor characteristics in largemouth bass (Micropterus salmoides). Aquaculture, 510, 100–108. https://doi.org/10.1016/j.aquaculture.2019.05.051

Hossain, A., Badiuzzaman, Nielsen, M., & Roth, E. (2022). Consumer willingness to pay for quality attributes of pangasius (Pangasianodoan hypophthalmus) in Bangladesh: A hedonic price analysis. Aquaculture, 555, 738205. https://doi.org/10.1016/j.aquaculture.2022.738205

Huy, D.T.N., Nam, V.Q., Hanh, H.T., Minh, P.N., & Huong, L.T.T. (2022). A review and further analysis on seafood processing and the development of the fish Pangasius from the food industry perspective. Food Science and Technology, 42, e76421. https://doi.org/10.1590/fst.76421

Islam, S., Haque, M., Razzak, M., Schlüter, L., Ahsan, E., Podduturi, R., & Jørgensen, N. (2021). Yellow tainting of flesh in pangasius (Pangasianodon hypophthalmus): Origin of the color and procedures for removal. Aquatic Sciences and Engineering, 36(4), 193–201. https://doi.org/10.26650/ASE2021862649

Junior, G.B., Bianchini, A.E., De Freitas Souza, C., Descovi, S.N., Da Silva Fernandes, L., De Lima Silva, L., Cargnelutti, J.F., & Baldisserotto, B. (2022). The use of cinnamon essential oils in aquaculture: Antibacterial, anesthetic, growth-promoting, and antioxidant effects. Fishes, 7(3), 133. https://doi.org/10.3390/fishes7030133

Laheng, S., Setiawati, M., Jusadi, D., & Agus Suprayudi, M. (2016). Applications of the addition of extract and cinnamon leaf flour in the diet on the quality of meat of catfish. Jurnal Pengolahan Hasil Perikanan Indonesia, 19(1), 36–43. https://doi.org/10.17844/jphpi.2016.19.1.36

Li, J.-E., Futawaka, K., Yamamoto, H., Kasahara, M., Tagami, T., Liu, T.-H., & Moriyama, K. (2015). Cinnamaldehyde contributes to insulin sensitivity by activating PPARδ, PPARγ, and RXR. The American Journal of Chinese Medicine, 43(05), 879–892. https://doi.org/10.1142/S0192415X15500512

Li, J.-M., Li, L.-Y., Qin, X., Ning, L.-J., Lu, D.-L., Li, D.-L., Zhang, M.-L., Wang, X., & Du, Z.-Y. (2017). Systemic regulation of L-carnitine in nutritional metabolism in zebrafish, Danio rerio. Scientific Reports, 7(1), 40815. https://doi.org/10.1038/srep40815

Li, J.-M., Zhang, Z., Kong, A., Lai, W., Xu, W., Cao, X., Zhao, M., Li, J., Shentu, J., Guo, X., & Mai, K. (2023). Dietary L-carnitine regulates liver lipid metabolism via simultaneously activating fatty acid β-oxidation and suppressing endoplasmic reticulum stress in large yellow croaker fed with high-fat diets. British Journal of Nutrition, 129(1), 29–40. https://doi.org/10.1017/S0007114522000101

Li, L., Limbu, S. M., Ma, Q., Chen, L., Zhang, M., & Du, Z. (2019). The metabolic regulation of dietary L‐carnitine in aquaculture nutrition: Present status and future research strategies. Reviews in Aquaculture, 11(4), 1228–1257. https://doi.org/10.1111/raq.12289

Nhu, T.T., Schaubroeck, T., De Meester, S., Duyvejonck, M., Sorgeloos, P., & Dewulf, J. (2015). Resource consumption assessment of Pangasius fillet products from Vietnamese aquaculture to European retailers. Journal of Cleaner Production, 100, 170–178. https://doi.org/10.1016/j.jclepro.2015.03.030

Orban, E., Nevigato, T., Lena, G.D., Masci, M., Casini, I., Gambelli, L., & Caproni, R. (2008). New trends in the seafood market Sutchi catfish (Pangasius hypophthalmus) fillets from Vietnam: Nutritional quality and safety aspects. Food Chemistry, 110, 383–389. https://doi.org/10.1016/j.foodchem.2008.02.014

Phuong, L.M., Huong, D.T.T., Malte, H., Nyengaard, J.R., & Bayley, M. (2018). Ontogeny and morphometrics of the gill and swim bladder of air-breathing striped catfish Pangasianodon hypophthalmus. Journal of Experimental Biology, 221(3), jeb.168658. https://doi.org/10.1242/jeb.168658

Pietsch, C., Schulz, C., Rovira, P., Kloas, W., & Burkhardt-Holm, P. (2014). Organ damage and hepatic lipid accumulation in carp (Cyprinus carpio L.) after feed-borne exposure to the mycotoxin, deoxynivalenol (DON). Toxins, 6(2), 756–778. https://doi.org/10.3390/toxins6020756

Platas-Rosado, D.E., González-Reynoso, L., Hernández-Arzaba, J.C., & Torres-Tadeo, C.M. (2021). Economic impact and feasibility of striped catfish farming (Pangasius hypophthalmus) in Mexico. Agro Productividad, 14(4), 155–159. https://doi.org/10.32854/agrop.v14i4.2015

Rathod, N.B., Pagarkar, A.U., Pujari, K.H., Shingare, P.E., Satam, S.B., Phadke, G. G., & Gaikwad, B.V. (2018). Status of valuable components from Pangasius: A review. International Journal of Current Microbiology and Applied Sciences, 7(04), 2106–2120. https://doi.org/10.20546/ijcmas.2018.704.241

Ravardshiri, M., Bahram, S., Javadian, S.R., & Bahrekazemi, M. (2021). Cinnamon promotes growth performance, digestive enzyme, blood parameters, and antioxidant activity of rainbow trout (Oncorhynchus mykiss) in low-carbohydrate diets. Turkish Journal of Fisheries and Aquatic Sciences, 21(07), 309–322. https://doi.org/10.4194/1303-2712-v21_7_01

Rincón, L., Castro, P.L., Álvarez, B., Hernández, M.D., Álvarez, A., Claret, A., Guerrero, L., & Ginés, R. (2016). Differences in proximal and fatty acid profiles, sensory characteristics, texture, colour and muscle cellularity between wild and farmed blackspot seabream (Pagellus bogaraveo). Aquaculture, 451, 195–204. https://doi.org/10.1016/j.aquaculture.2015.09.016

Rolin, F., Setiawati, M., & Jusadi, D. (2015). Evaluasi pemberian ekstrak daun kayu manis Cinnamomum burmannii pada pakan terhadap kinerja pertumbuhan ikan patin Pangasianodon hypophthalmus Sauvage, 1878. Jurnal Iktiologi Indonesia, 15(3), 201–208. https://dx.doi.org/10.32491/jii.v15i3.56

Roques, S., Deborde, C., Richard, N., Skiba‐Cassy, S., Moing, A., & Fauconneau, B. (2020). Metabolomics and fish nutrition: A review in the context of sustainable feed development. Reviews in Aquaculture, 12(1), 261–282. https://doi.org/10.1111/raq.12316

Salmerón, C. (2018). Adipogenesis in fish. Journal of Experimental Biology, 221, 1–11. https://doi.org/10.1242/jeb.161588

Sanchez, M.S.D.S., Lins-Rodrigues, M., Pessini, J.E., Bittencourt, F., Boscolo, W. R., & Signor, A. (2021). Dietary supplementation with l-carnitine for Nile tilapia juveniles. Aquaculture, 539, 736616. https://doi.org/10.1016/j.aquaculture.2021.736616

Schram, E., Kwadijk, C., Hofman, A., Blanco, A., Murk, A., Verreth, J., & Schrama, J. (2021). Effect of feeding during off-flavour depuration on geosmin excretion by Nile tilapia (Oreochromis niloticus). Aquaculture, 531, 735883. https://doi.org/10.1016/j.aquaculture.2020.735883

Setiawati, M., Jusadi, D., Laheng, S., Suprayudi, M.A., & Vinasyiam, A. (2016). The enhancement of growth performance and feed efficiency of Asian catfish, Pangasianodon hypophthalmus fed on Cinnamomum burmannii leaf powder and extract as nutritional supplementation. AACL Bioflux, 9(6), 1301–1309.

Setiawati, M., Sakinah, A., & Jusadi, D. (2015). Evaluation of growth and meat performance of Pangasianodon hypopthalmus which fed with Cinnamomum burmanni leaves enriched diet. Jurnal Akuakultur Indonesia, 14(2), 171–178. https://doi.org/10.19027/jai.14.171-178

Siagian, D.R., Jusadi, D., Ekasari, J., & Setiawati, M. (2021). Dietary α-lipoic acid supplementation to improve growth, blood chemistry, and liver antioxidant status of African catfish Clarias gariepinus. Aquaculture International, 29(5), 1935–1947. https://doi.org/10.1007/s10499-021-00726-1

Sugata, M., Wiriadi, P.F., Lucy, J., & Jan, T.T. (2019). Total lipid and omega-3 content in Pangasius catfish (Pangasius pangasius) and milkfish (Chanos chanos) from Indonesia. Malaysian Journal of Nutrition, 25(1), 163–169. https://doi.org/10.31246/mjn-2018-0137

Suryaningrum, T.D., Muljanah, I., & Tahapari, E. (2010). Profil sensori dan nilai gizi beberapa jenis ikan patin dan hibrid nasutus. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan, 5(2), 153. https://doi.org/10.15578/jpbkp.v5i2.419

Suwarsito. (2007). Pengaruh L-Karnitin terhadap kadar lemak daging dan komposisi tubuh ikan patin (Pangasius hypophthalmus). Journal of Fisheries Sciences, 9(1), 63–68.

Tartila, S.S.Q., Jusadi, D., Setiawati, M., & Fauzi, I.A. (2021). Evaluation of dietary supplementation with cinnamon products on growth, blood composition, liver structure, and meat quality of striped catfish (Pangasianodon hypophthalmus). Aquaculture International, 29(5), 2243–2257. https://doi.org/10.1007/s10499-021-00746-x

Varner, W. (2000). Anesthetics. In R.R. Stickney (Ed.), Encyclopedia of Aquaculture (pp. 33–38). John Wiley & Sons, Inc.

Wahyudi, I.T., Jusadi, D., Setiawati, M., & Ekasari, J. (2023). Effects of dietary supplementation with cinnamon powder and lysine on blood chemistry, liver histology, growth performance, and fillet quality of striped catfish Pangasianodon hypophthalmus. Aquaculture International, 31, 3513–3529. https://doi.org/10.1007/s10499-023-01141-4

Watanabe, T. (1998). Fish Nutrition and Mariculture. Japan International Cooperation Agency.

Wisudawaty, P., Yuliasih, I., & Haditjaroko, L. (2020). Aplikasi edible coating minyak kayu manis pada manisan tomat cherry selama penyimpanan. Jurnal Teknologi Industri Pertanian, 30(1), 63–71. https://doi.org/10.24961/j.tek.ind.pert.2020.30.1.63

Zhu, R., Liu, H., Liu, C., Wang, L., Ma, R., Chen, B., Li, L., Niu, J., Fu, M., Zhang, D., & Gao, S. (2017). Cinnamaldehyde in diabetes: A review of pharmacology, pharmacokinetics and safety. Pharmacological Research, 122, 78–89. https://doi.org/10.1016/j.phrs.2017.05.019




DOI: http://dx.doi.org/10.15578/jra.18.1.2023.1-14


Lisensi Creative Commons
Jurnal Riset Akuakultur is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

View My Stats
p-ISSN 1907-6754
e-ISSN 2502-6534