PENGARUH PEMBERIAN HORMON PERTUMBUHAN REKOMBINAN DENGAN METODE YANG BERBEDA TERHADAP PERTUMBUHAN BENIH IKAN BOTIA (Chromobotia macracanthus)

Asep Permana, Alimuddin Alimuddin, Wartono Hadie, Agus Priyadi, Rendy Ginanjar

Abstract


Pertumbuhan ikan botia tergolong lambat, memerlukan waktu sekitar enam bulan untuk mencapai ukuran komersial (panjang total 4-5 cm). Penelitian ini bertujuan untuk mengevaluasi respons pertumbuhan benih ikan botia yang diberi hormon pertumbuhan ikan kerapu (rElGH) melalui tiga metode yaitu: perendaman, oral, dan kombinasi perendaman dan oral. Penelitian menggunakan rancangan acak lengkap dan setiap perlakuan diulang tiga kali. Dosis rElGH melalui perendaman yaitu 1,2 mg/L diberikan pada larva umur tujuh hari, sedangkan dosis secara oral yaitu 30 mg/kg pakan dan diberikan pada benih umur tiga bulan. Ikan dipelihara dalam akuarium sistem resirkulasi (80 cm x 40 cm x 25 cm) dengan kepadatan 5 ekor/L selama pemeliharaan tiga bulan pertama dan 1 ekor/L selama pemeliharaan periode tiga bulan kedua. Hasil penelitian menunjukkan bahwa perlakuan kombinasi perendaman dan oral memberikan pertumbuhan yang lebih tinggi sebesar 12,04% dibandingkan kontrol. Perlakuan ini juga meningkatkan level ekspresi insulin-like growth factor-1/ IGF-1 sebesar 29,37% dibandingkan kontrol.

The growth of Clown Loach is slow. It takes about six months to reach the market size (4-5 cm total body length). This study aimed to evaluate the growth response of Clown Loach treated with recombinant giant grouper growth hormone (rElGH) delivered by three different methods: immersion, oral, and the combination of immersion and oral. A completely randomized design was used as the experimental design and each treatment was replicated three times. The immersion method used rElGH dose of 1.2 mg/L on seven-day-old larvae, while oral treatment used 30 mg rElGH/kg feed on the three-month-old juvenile. The fish were reared in a closed recirculation tank (80 cm x 40 cm x 25 cm) at a density of 5 fish/L for the first three months, and 1 fish/L for the second three months of rearing period. The result showed that the combination of immersion and oral treatments produced a higher growth rate of 12.04% compared to control treatment. Those treatments also increased insulin-like growth factor-1/IGF-1 expression level about 29.37% compared to the control.


Keywords


recombinant growth hormone; metode pemberian hormon; Chromobotia macracanthus; recombinant growth hormone; delivery method; clownloach; Chromobotia macracanthus

Full Text:

PDF


DOI: http://dx.doi.org/10.15578/jra.13.2.2018.123-130


Lisensi Creative Commons
Jurnal Riset Akuakultur is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

View My Stats
p-ISSN 1907-6754
e-ISSN 2502-6534


Find in a library with WorldCatCrossref logoSHERPA/RoMEO LogoHasil gambar untuk isjdgoogle scholardoaj