PREVALENCE OF IRIDOVIRUS AND VIRAL NERVOUS NECROSIS IN CANTANG GROUPER (Ephinephelus fuscogottatus x Ephinephelus lanceolatus)

Fadia Tantri Alfianti; Dzikri Wahyudi; Prasetya Wahyu Kusuma; Wahyu Budi Wibowo; Guntur Prabowo; Achmad Suhermanto

doi:10.15578/aj.v6i2.13467

PREVALENCE OF IRIDOVIRUS AND VIRAL NERVOUS NECROSIS IN CANTANG GROUPER (Ephinephelus fuscogottatus x Ephinephelus lanceolatus)

Fadia Tantri Alfianti, Dzikri Wahyudi, Prasetya Wahyu Kusuma, Wahyu Budi Wibowo, Guntur Prabowo, Achmad Suhermanto

Abstract

Cantang grouper is a leading commodity, especially hatchery centers, namely East Java and Bali which is Indonesia's flagship. The growth of cantang grouper fish is hampered by one of the diseases caused Iridovirus and Viral Nervous Necrosis (VNN). These two diseases attacks the seed stage which causes high mortality, so that early detection is needed for preventive action. This study aims to detect and inventory of Iridovirus and VNN in cantang grouper. This research was carried out at BPBAP Situbondo, Irodovirus was detected using conventional PCR and VNN using rRT-PCR. Cantang grouper samples measuring 2,5-12 cm came from Situbondo and its surroundings, Bali and Lombok with a total of 23 samples. The test results showed that all samples were negative for Iridovirus, while the VNN examination showed 3 positive samples and 20 negative samples. Observation of clinical symptoms showed that the cantang grouper were in good health, swimming actively and did not show symptoms of Iridovirus and VNN infection. The prevalence of VNN infection in cantang grouper 13,04% which was included in the category of frequent infections.

Keywords

Cantang, Diseases, Iridovirus, Situbondo, Viral Nervous Necrosis

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References

Bachtiar. (2020). Infeksi Megalocytivirus Pada Budidaya Ikan Air Laut Dan Ikan Air Tawar Di Beberapa Provinsi Di Indonesia. Jurnal Veteriner, Vol. 21 No. 3 : 423-434.

Chinchar, V. G., Hyatt, A., Miyazaki, T., & Williams, T. (2009). Family Iridoviridae: poor viral relations no longer. Lesser known large dsDNA viruses, 123-170.

Hapsari, I. (2022). Viral Nervous Necrosis (VNN). Balai Karantina Ikan Pengendalian Mutu Dan Keamanan Hasil Perikanan.

Hazreen-Nita, M., Azila, A., Mukai, Y., Firdaus-Nawi, M., & Nur-Nazifah, M. (2019). A review of betanodavirus vaccination as preventive strategy to viral nervous necrosis (VNN) disease in grouper. Aquaculture International, 27(5), 1565-1577.

Khumaidi, A., Fadjar, M., Iranawati, F., Kilawati, Y., & Yanuhar, U. (2019). Mass mortality associated with viral nervous necrosis of hybrid grouper (Epinephelus sp.) cultured in city of grouper. In AIP Conference Proceedings (Vol. 2120, No. 1). AIP Publishing.

Mao, M. G. (2015). Evidence For And Characterization Of Nervous Necrosis Virus Infection In Pacific Cod (Gadus macrocephalus). Arch Virol, 160:2237–2248.

OIE. (2009). Manual diagnostic test for aquatic animals. chapter 2.3.7 red sea bream iridoviral disease. Fish Pathology, 52(2), 57–62. https://doi.org/10.3147/jsfp.52.57

Othman, A. R. (2015). Effects of Different Salinities on Growth, Feeding Performance and Plasma Cortisol Level in Hybrid (Tiger Grouper, Epinephelus fuscoguttatus x giant Grouper, Epinephelus lanceolatus) Juveniles. International Research Journal Of Biological Sciences, 15-20.

Rafael, J. F., & Roza, D. (2009). Kasus Infeksi Virus Irido pada Benih Ikan Kerapu Pasir, Epinephelus Corallicola di Hatchery. Jurnal Perikanan 8-12.

Razak, AA, Ransangan J, Sade, A (2014) First report of megalocytivirus (Iridoviridae) in grouper culture in Sabah, Malaysia. Int J Curr Microbiol Appl Sci 3(3):896–909 .

Rimmer, M. A., Whittington, R. J., Becker, J. A., Dhand, N. K., Hick, P. M., Mahardika, K., Sembiring, S., Ismi, S., Zafran, Z., Haryanti, Roza, D., Kusumawati, D., Asih, Y. N., Sudewi, Mastuti, I., Wardana, I. K., Murwantoko, Fusianto, C. K., & Fachry, M. E. (2019). Improving fish health management and production protocols in marine finfish aquaculture in Indonesia and Australia. Project number: ACIAR/FIS/2010/101.

Sembiring, S. B. (2018). Prevalensi Infeksi Viral Nervous Necrosis (VNN) Dan Iridovirus Pada Hatcheri dan Budidaya Ikan Laut. Media Akuakultur, 83-90.

Susanti, E. (2016). Identeksi Virus TSV (Taura Syndrome Virus ) pada Udang Vannamei (Litopenaeus vannamei) Di Kabupaten Mempawah Hilir Dengan Metode PCR (Polymerase Chain Reuction). Skripsi. Universitas Muhammadiyah Pontianak.

Wahyudi, D., Suhermanto, A., Atri Triana, K., & Herdianto, T. (2022). Deteksi Parasit Dan Virus pada Pembesaran Ikan Kerapu Cantang (Epinephelus fuscoguttatus vs Epinephelus lanceolatus). Jurnal Airaha, 11(02).

Wei, J., Huang, Y., Zhu, W., Li, C., Huang, X., & Qin, Q. (2019). Isolation and identification of Singapore grouper iridovirus Hainan strain (SGIV-HN) in China. Archives of virology, 164, 1869-1872.

Williams, E. H. Jr. & L.B. Williams. (1996). Parasites of offshore big game fishes of Puerto Rico and the western Atlantic. Puerto Rico: Departement of Natural and Environmental Resources University of Puerto Rico.

Widiartini. (2022). Karakteristik Gejala Klinis Dan Dampak yang Ditimbulkan oleh Parasit Oodinium sp. pada Ikan Kerapu Cantang yang Dibudidayakan Dengan Sistem Recirculating Aquaculture System (RAS). Undergraduate Thesis. Universitas Pendidikan Ganesha.

Xiao, H., Liu, M., Li, S., Shi, D., Zhu, D., Ke, K., ... & Li, P. (2019). Isolation and characterization of a ranavirus associated with disease outbreaks in cultured hybrid grouper (♀ Tiger Grouper Epinephelus fuscoguttatus×♂ giant grouper E. lanceolatus) in Guangxi, China. Journal of Aquatic Animal Health, 31(4), 364-370.

Yu, Q., Liu, M., Wei, S., Xiao, H., Wu, S., Qin, X., ... & Li, P. (2019). Isolation of nervous necrosis virus from hybrid grouper (Epinephelus fuscoguttatus♀× Epinephelus lanceolatus♂) cultured in Guangxi, China. Fish Pathology, 54(1), 16-19.

DOI: http://dx.doi.org/10.15578/aj.v6i2.13467