VARIASI GENETIKA IKAN BANYAR, Rastrelliger kanagurta (Cuvier, 1817) DI PERAIRAN INDONESIA TIMUR

Achmad Zamroni, Suwarso Suwarso, Adi Kuswoyo

Abstract


Ikan banyar, Rastrelliger kanagurta (Cuvier, 1817) merupakan salah satu jenis ikan pelagis kecil bernilai ekonomis tinggi. Di perairan Indonesia bagian timur ikan ini mendominasi hasil tangkapan, terutama di sekitar Papua. Indikasi adanya penangkapan yang semakin intensif menyebabkan turunnya hasil tangkapan ikan banyar akibat turunnya kemampuan ikan untuk bereproduksi. Populasi yang berkurang dapat munurunkan variasi genetika yang ada. Untuk itu diperlukan suatu kajian variasi genetika ikan banyar. Penelitian ini menggunakan RAPD (Amplified Fragment Length Polymorphism) dengan lima primer. Sampel daging dari 24 ekor ikan dikumpulkan dari lima lokasi: Gorontalo, Kendari, Ambon, Raja Ampat dan Dobo. Hasil penelitian menunjukkan bahwa variasi genetika ikan banyar di Indonesia timur terdiri dari dua kelompok utama, kelompok pertama diwakili populasi Raja Ampat, Ambon, Kendari dan Gorontalo, kelompok kedua populasi dari Dobo.

 

Indian mackerel, Rastrelliger kanagurta (Cuvier, 1817) is an economic important small pelagic species. In the eastern part of Indonesia this species dominates the catch, especially surrounding of Papua Waters. Highly intensive catch activity alleges the catch down due to reducing the reproduction capasity and genetic variation. Therefore, a study of genetic variations of Indian mackerel fish in the waters of Eastern Indonesia is required. The research used RAPD (Amplified Fragment Length Polymorphism) with five primers. Twenty four sample of the muscle were collected from Gorontalo, Kendari, Ambon, Raja Ampat, and Dobo. The results showed that genetic variation consisted of two main groups. The first group consistinged of Raja Ampat, Ambon, Kendari and Gorontalo populations, and the second group was the population from Dobo.


Keywords


RAPD; genetika; ikan banyar; Rastrelliger; Indonesia

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References


Akbar, N., Zamani, N. P., & Madduppa, H. W. (2014). Keragaman genetik ikan tuna sirip kuning (Thunnus albacares) dari Dua Populasi di Laut Maluku, Indonesia. Depik, 3(1), 65-73.

Akib, N. A. M., Tam, B. M., Phumee, P., Abidin, M. Z., Tamadoni, S., Mather, P. B., & Nor, S. A. M. (2015). High connectivity in Rastrelliger kanagurta: Influence of Historical Signatures and Migratory Behaviour Inferred from mtDNA Cytochrome b. PLoS ONE, 10(3).

Allendorf, F.W., England, P. R., Luikart, G., & Ritchie, P. A. (2008). Genetic effects of harvest on wild animal populations. Trends in Ecol. Evol., 23, 327-337.

Bermingham, E. (1990). Mitochondrial DNA and the Analysis of Fish Population Structure. In: D. H. Whitmore (ed.), Electrophoretic and Isoelectric Focusing Techniques in Fisheries Management (pp. 107-129). Florida, FL: CRC Press.

Chen, Y., Cheng, Q., Qiao, H., Zhu, Y., & Chen, W. (2013). The complete mitochondrial genome sequence of Rastrelliger kanagurta (Perciformes: Scombridae). Mitochondrial DNA, 24(2). 114-116.

Chiang, H.C., Hsu, C. C., Lin, H.D., Ma, G.C., Chiang, T.Y., & Yang, H.Y. (2006.) Population structure of bigeye tuna (Thunnus obesus) in the South China Sea, Philippine Sea and western Pacific Ocean inferred from mitochondrial DNA. Fisheries Research. 79, 219–22 5.

Chiang, H.C., Hsu, C.C., Wu, G.C.C., Chang, S.K., & Yang, H.Y. (2008). Population structure of bigeye tuna (Thunnus obesus) in the Indian Ocean inferred from mitochondrial DNA. Fisheries Research. 90, 305-312.

Darlina, M. N., Masazurah, A. R., Jayasankar, P., Jamsari, A. F. J., & Siti, A. M. N. (2011). Morphometric and molecular analysis of mackerel (Rastrelliger spp) from the west coast of Peninsular Malaysia. Genetics and Molecular Research, 10(3), 2078-2092.

Daruwedho, H., Sasmito, B., & Fauzi, J. A. (2016). Analisis pola arus laut permukaan perairan Indonesia Menggunakan Satelit Altimetri Jason-2 Tahun 2010-2014. Jurnal Geodesi Undip, 5(2), 145-158.

DJPT. (2016). Statistik perikanan tangkap Indonesia, 2015.

Fakhri, F., Narayani, I., & Mahardika, I. G. N. K. (2015). Keragaman genetik ikan cakalang (Katsuwonus pelamis) dari Kabupaten Jembrana dan Karangasem, Bali. Jurnal Biologi, 19(1).

Gaylord, B. & Gaines, S. D. (2000). Temperature or transport? Range limits in marine species mediated solely by flow. American Naturalist, 155, 769-789.

Giles, J. L., Ovenden, J. R., Dharmadi, D., AlMojil, D., Garvilles, E., Khampetch, K., Manjebrayakath, H., & Riginos, C. (2014). Extensive genetic population structure in the Indo–West Pacific spot-tail shark, Carcharhinus sorrah. Bulletin of Marine Science, 90(1). 427-454.

Gordon, A. L. & Fine, R. A. (1996). Pathways of water between the Pacific and Indian oceans in the Indonesian seas. Nature, 379, 146–149.

Hartl, D. L. & Jones, E. W. (1998). Genetics: principles and analysis (4th ed.). United State of America: Jones and Bartlett Publishers. Inc.

Hedrick, P. W. (2000). Genentics of populations (2nd ed.). Sudbury: Jones and Bartlett Publishers.

Hughes, A. R., Inouye, B. D., Johnson, M. T. J., Underwood, N., & Vellend, M. (2008). Ecological consequences of genetic diversity. Ecology Letters, 11, 609-623.

Indaryanto, F. R., Imai, H., & Wardiatno, Y. (2015). Genetic variation of short body mackerel, Rastrelliger brachysoma of Jawa Island, Indonesia based on mtDNA control region sequences. AACL Bioflux, 8(5), 648-655.

Jayasankar, P., Thomas, P. C., Paulton, M. P., & Mathew, J. (2004). Morphometric and genetic analyzes of indian mackerel (Rastrelliger kanagurta) from Peninsular India. Asian Fisheries Science, 17. 201-215.

Jondeung, A & Karinthanyakit, W. (2015). Mitochondrial DNA control region of three mackerels, genus Rastrelliger: structure, molecular diversity and phylogenetic relationship. Mitochondrial DNA, Early Online. 1-6.

Kielpinski, M., Kempter, J., Panicz, R., & Keszka, S. (2014). Genetic identifiability of selected populations of indian mackerel, Rastrelliger kanagurta (Actinopterygii: Perciformes: Scombridae) – Celfish Project – Part 1. Acta Ichthyol. Pascat, 44(2), 145-152.

Kumar, N. & Thakur, S. K. (2014). Randomly amplified polymorphic DNA-A Brief Review. American Journal of Animal and Veterinary Sciences, 9(1), 6-13.

Menezes, M. R., Naik., S. & Martins, M. (1990). Genetic and morphological variations in the Indian mackerel Rastrelliger kanagurta (Cuvier 1817) from the Goa region. Proc. Indian Acad. Sci., 99(6), 457-465.

Menezes, M. R., Naik, S., & Martins, M. (1993). Genetic divergence in the Indian Mackerel Rastrelliger kanagurta (Cuv) from the coastal waters of Peninsular India and the Andaman Sea. Indian Journal of Fisheries, 40(3). 135-141.

Miller, M. P. (1997). Tools for Population Genetic Analysis (TFPGA). Version 1.3. Department of Biological Sciences, Northern Arizona University, Flagstaff.

Moria, S. B., Haryanti, H., Permana, G. N., & Slamet, B. (2006). Karakteristik genetik dan struktur populasi ikan napoleon, Cheilinus undulates di Perairan Indonesia. Jurnal Riset Akuakultur. 1(3), 315-323.

Nei, M. & Tajima, F. (1981). DNA polymorphism detectable by restriction endonucleases. Genetics. 97, 145 – 163.

Nishida, T., Chow, S., & Grewe, P. (1998). Review and research plan on the stock sructure of yellowfin tuna (Thunnus albacares) and bigeye tuna (Thunnus obesus) in the Indian Ocean. Proceedings Indian Ocean Tuna Commission, Victoria, Seychelles. 9-14 November 1998.

Reynolds, J., Weir, B. S., & Cockerham, C. C. (1983). Estimation of the coancestry coefficient: basis for a short-term genetic distance. Genetics, 105(3), 767-779.

Santos, M. D., Lopez, G. V., & Baru, N. C. (2010). A pilot study on the genetic variation of eastern little tuna (Euthynnus affinis) in Southeast Asia. Philippine J. of Sci. 139(1), 43-50.

Sukumaran, S., Sebastian, W., & Gopalakrishnan, A. (2017). Genetic population structure and historic demography of Indian mackerel, Rastrelliger kanagurta from Indian peninsular waters. Fisheries Research, 191. 1-9.

Suwarso, S. & Zamroni, A. (2014). Analisis struktur populasi tiga spesies layang (Decapterus spp.) di Laut Jawa dan sekitar Sulawesi: Saran Pengelolaan Berkelanjutan Ikan Pelagis Kecil dan Evaluasi WPP. Jurnal Kebijakan Perikanan Indonesia, 6(2), 75 – 86.

Tabata, K. H., Kishioka, M., Takagi, A., Mizuta, N., & Taniguchi, T. (1997). Genetic diversity of five strains of red sea bream Pagrus major by RFLP analysis of the mtDNA D-Loop region. Journal Fisheries Science. 63(3), 344-348.

Willette, D. A., Santos, M. S. & Leadbitter, D. (2016). Longtail tuna Thunnus tonggol (Bleeker, 1851) shows genetic partitioning across, but not within, basins of the Indo-Paciûc based on mitochondrial DNA. Journal of Applied Ichthyology. 32, 318-323.

Wilson, D. S., & Clarke, A. B. (1996). The shy and the bold. Natural History. 9/96, 26–28.

Wright, S. (1951). The genetical structure of populations. Annals of Eugenics. 15(4), 323-354.

Wyrtki, K. (1961). Physical oceanography of the Southeast Asian Water. NAGA Report, Scripps Inst. Oceanography. The University of California, La Jolla, California. 2.

Zamroni, A., Suwarso, S., & Nugroho, E. (2014). Struktur genetika populasi ikan malalugis biru (Decapterus macarellus Cuvier, 1833) Di Sekitar Sulawesi Berdasarkan Mt-DNA Marker. J. Lit. Perik. Ind. 20(1), 31-34.

Zamroni, A., Suwarso, S. & Mardlijah, S. (2016). Genetika populasi Rastrelliger kanagurta Di Perairan Barat Sumatera, Selat Malaka Dan Laut Cina Selatan. J. Lit. Perik. Ind., 22(1), 1-8.

Zein, M. S. A. (2007). Keragaman daerah kontrol DNA Mitokondria Rusa Timor (Cervus timorensis timorensis) di Pulau Timor, Alor dan Pantar. Biota, 12(3), 138-144.




DOI: http://dx.doi.org/10.15578/bawal.9.2.2017.123-131


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