ANALISIS KANDUNGAN SENYAWA FENOLIK EKSTRAK ALGA Sargassum polycystum DARI PANTAI SELATAN, GUNUNG KIDUL, YOGYAKARTA

Radipta - Lailatussifa; Maria Madalena Pereira

doi:10.15578/chanos.v20i1.10532

ANALISIS KANDUNGAN SENYAWA FENOLIK EKSTRAK ALGA Sargassum polycystum DARI PANTAI SELATAN, GUNUNG KIDUL, YOGYAKARTA

Radipta - Lailatussifa, Maria Madalena Pereira

Abstract

Sargassum sp. is one of the abundant brown algae on the South Coast of Yogyakarta. This algae has pharmacological activity in the form of metabolite biological activity, one of which is as an antioxidant. Regarding its ability as an antioxidant, information is needed regarding the polyphenol content in the algae S. polycystum. The aims from this research were to determine of phenolic content analysis of Sargassum polycystum brown algae from South Beach, Gunung Kidul, Yogyakarta quantitatively related to its total phenolic content and qualitatively consisting of Thin Layer Chromatography (TLC) and HPLC (High Performance Liquid Chromatography) tests. Polyphenol was extracted with ethanol 96% and calculated the yield. The yield from fresh algae to powdered algae was 9%, while the yield from fresh algae to ethanolic extract was 5.1%. The value of the total phenolic content extract at a concentration of 1000 ppm was 59.30±0,58 g GAE/100 g dried extract. The TLC test was performed using gallic acid and phloroglucinol standard, while the HPLC test was performed using gallic acid, phloroglucinol and phenol standard. The TLC dan HPLC results showed that ethanolic extract of Sargassum polycystum was suspected to contain polyphenols that resembled of gallic acid and phenol standards.

Keywords

ethanolic extract, High Performance Liquid Chromatography, Sargassum polycystum, Thin Layer Chromatography, Total Phenolic Content

Full Text:

PDF

References

Boonchum, W., Y. Peerapornpisal, D. Kanjanapothi, J. Pekkoh, C. Pumas, U. Jamjai. 2011. Antioxidant Activity of Some Seaweed from the Gulf of Thailand. International Journal of Agriculture and Biology, 13: 95-99.

Farvin, K. H. S., & Charlotte Jacobsen. 2013. Phenolic Compounds and Antioxidant Activities of Selected Species of Seaweeds from Danish Coast. Journal Food Chemistry. 138: 1670-1681.

Firdaus, M. 2011. Aktivitas Antioksidan Ekstrak Rumput Laut Coklat (Sargassum echinocarpum) sebagai Pencegah Disfungsi Sel Endothelium Aorta Tikus Diabetes Mellitus. Disertasi Institut Pertanian Bogor. Bogor.

Gazali, M., Nurjanah, N.P. Zamani. 2018. The exploration of bioactive compound to brown algae Sargassum sp. Agardh as antioxidant from west of Aceh Coastal. JPHPI, 21(1): 167-178.

Heo, S. J., E.J. Park, K.W. Lee, Y. J. Jeon. 2005. Antioxidant Activities of Enzymatic Extracts from Brown Seaweed. Bioresource Technology. 96(14): 1613–1623.

Holdt, S. L. and K. Stefan. 2011. Bioactive Compounds in Seaweed : Fuctional Food Applications and Legislation. Journal Application Phycology. 23 : 543-597.

Husni, A., R. Wijayanti, Ustadi. 2014. Inhibitory activity of α-amilase and α-glucosidase by Padina pavonica extracts. Journal of Biological Sciences, 14(8): 515.

Husni, A., T. Pratiwi, Ustadi, A.G. Samudra, A.E. Nugroho. 2018. In vitro antidiabetic activity of Sargassum hystrix and Eucheuma denticulatum from Yogyakarta Beach of Indonesia. Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, 55(3): 1-8.

Kang, C., Y.B. Jin, H. Lee, M.Cha, E. Sohn, J. Moon, C. Park, S. Chun, E. Jung, J. Hong, S.B. Kim, J. Kim, E. Kim. 2010. Brown Alga Ecklonia cava Attenuates Type 1 Diabetes by Activating AMPK and Akt Signaling Pathways. Journal Food and Chemical Technology, 48: 509-516.

Khadijah, A.M. Jayali, S. Umar, I. Sasmita. 2017. Penentuan total fenolik dan aktivitas antioksidan ekstrak etanolik daun samama (Anthocephalus macrophylus) Asal Ternate, Maluku Utara. Jurnal Kimia Mulawarman, 15(1): 11-18.

Kuda, T., Tsunekawa, M. Goto, H. Araki. 2005. Antioxidant properties of four edible algae harvested in the Noto Peninsula, Japan. Journal of Food Composition and Analysis. 18: 625–633.

Lann, K. L., C. Ferret, E. VanMee, C. Spagnol, M. Lhuillery, C. Payri, V. Stiger-Pouvreau. 2012. Total Phenolic, Size-Fractioned Phenolics and Fucoxanthin Content of Tropical Sargassaceae (Fucales, Phaeophyceae) from the South Pacific Ocean: Spatial and Specific Variability. Journal Phycologycal Research, 60: 37-50.

Lantah, P.L., L.A.D.Y. Montolalu, A.R. Reo. 2017. Kandungan fitokimia dan aktivitas antioksidan ekstrak methanol rumput laut Kappaphycus alvarezii. Jurnal Media Teknologi Hasil Perikanan, 5(3): 73-79.

Lordan, S., R.P. Ross, C. Stanton. 2011. Marine Bioactives as Functional Food Ingredients: Potential to Reduce the Incidence of Chronic Diseases. Marine Drug. 9: 1056–1100.

Luo, H. Y., B. Wang, C.G. Yu, Y.I. Qu, G.I. Su. 2010. Evaluation of Antioxidant Activities of Five Selected Brown Seaweeds from China. Journal of Medicinal Plants Research, 4: 2557-2565.

Nagai, T. and T. Yukimoto. 2003. Preparation and functional Properties of Beverages Made from Sea Algae. Food Chemistry. 81: 327-332.

Plaza, M., M. Amigo-Benavent, M.D. del Castilo, E. Ibanez, M. Herrero. 2010. Facts about the Formation of New Antioxidants in Natural Samples after Subcritial Water Extraction. Food Research International. 43: 2341-2348.

Shibata, T., S. Kawaguchi, Y. Hama, M. Inagaki, K. Yamaguchi, and T. Nakamura. 2004. Local and Chemical Distribution of Phlorotannins in Brown Algae. Journal of Aplied Phycology, 16: 291-296.

Sorban, C., Moldoveanu, Victor David. 2013. Chapter 9- HPLC Analysis. Essentials in Modern HPLC Separations. p. 465-519.

You, T., and S. M. Barnett. 2004. Effect of Light Quality on Production of Extracellular Polysaccharides and Growth Rate of Porphyridium cruentum. Biochemical Engineering Journal, 19 : 251–258.

Zubia, M., D. Robledo, Y. Freile-Pelegrin. 2007. Antioxidant Activities in Tropical Marine Macroalgae from Yucatan Penisula, Mexico. Journal Application Phycology. 19 : 449-458.

DOI: http://dx.doi.org/10.15578/chanos.v20i1.10532