92 Citations
ISOLATION AND IDENTIFICATION OF PARTIAL GROWTH HORMONE (GH) mRNA OF TIGER SHOVELNOSE CATFISH Pseudoplatystoma fasciatum (LINNAEUS, 1766)
Abstract
The growth of fish is regulated by growth hormones secreted in a limited amount by its pituitary glands. Tiger shovelnose catfish has a relatively faster growth indicating that some growth hormones suspectedly play roles in the process and could be used in improving other farmed fish species. This study aimed to isolate and identify the growth hormone (GH) mRNA gene in Tiger shovelnose catfish (Pseudoplatystoma fasciatum Linnaeus, 1766). The sample was isolated from the pituitary, the fish measuring 602 g and 43 cm body length; Total RNA was extracted using the Tri Reagent Kit, followed with cDNA synthesis. The success of the extraction was confirmed by quantification and PCR using the β-actin gene as an internal control. GH mRNA gene was isolated by RT-PCR method, with degenerated primers from seven catfish species sequence data in the NCBI gene bank. The single band from GH gene was cloned and sequenced. Total RNA quantification with a concentration of 227 ng/µL and purity of 1.821. The successful isolation of mRNA from the pituitary gland was confirmed by amplifying the β-actin gene generated at 300 bp. This isolation of the GH mRNA gene had a sequence length of 234 bp. Tiger shovelnose catfish GH gene consists of 17 amino acid residues. The GH gene of Tiger shovelnose catfish was close to that of striped catfish (P. hypophthalmus) and Indian catfish (Clarias batrachus) with almost similar homology value of 90.6 %. Partial GH gene from tiger shovelnose catfish can be used a molecular marker in revealing the role of growth hormone on fish development, fish biology, growth gene expression, selective breeding and other mechanisms related to the aquaculture.
Keywords
Full Text:
PDFReferences
Abdolahnejad, Z., Pourkazemi, M., Khoshkholgh, M. R., & Yarmohammadi, M. (2015). Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii). Molecular Biology Research Communications, 4(4), 181–188. https://doi.org/10.22099/mbrc.2015.3166
Alimuddin, Handoyo, B., & Utomo, N. B. P. (2014). Effectiveness of recombinant giant grouper (Epinephelus lanceolatus, Bloch 1790) growth hormone through immersion and oral delivery on growth of eel (Anguilla bicolor bicolor). Jurnal Iktiologi Indonesia, 14(3), 179–189. https://doi.org/https://doi.org/10.32491/jii.v14i3.79
Alimuddin, Lesmana, I., Sudrajat, A. O., Carman, O., & Faizal, I. (2010). Production and Bioactivity Potential of Three Recombinant Growth Hormones of Farmed Fish. Indonesian Aquaculture Journal, 5(1), 11. https://doi.org/10.15578/iaj.5.1.2010.11-17
Alimuddin, Nugrahani, W., Aliah, R. S., & Sumantadinata, K. (2007). Isolation and characterization of B-actin promoter from humpback grouper (Cromileptes altivelis). Jurnal Riset Akuakultur, 2(2), 199–210. https://doi.org/http://dx.doi.org/10.15578/jra.2.2.2007.199-210
Apriliana, R., Basuki, F., & Agung, R. (2017). The effects of Recombinant Growth Hormone (rGH) with different doses for growth and survival of Java Barb Fish (Puntius sp.). Jurnal Sains Akuakultur Tropis, 2(July), 49–58. https://doi.org/https://doi.org/10.14710/sat.v2i1.2561
Britz, P., & Pienaar, A. (1992). Laboratory experiments on the efect of light and cover on the behaviour and growth of African catfish, Clarias gariepinus (Pisces: Clariidae). Journal of Zoology, 227, 43–62. https://doi.org/https://doi.org/10.1111/j.1469-7998.1992.tb04343.x
Buwono, I. D., Carsono, N., & Mulyani, Y. (2012). Isolation and analysis of growth hormone gene African catfish (Clarias gariepinus Burch). Jurnal Riset Akuakultur, 7(3), 371–379. https://doi.org/10.15578/jra.7.3.2012.371-379
Buwono, I. D., & Suparta, M. H. (2009). Isolation of partial fragments of growth hormone of Majalaya carp fish and GIFT nile using CTAB–PCR methods. Jurnal Riset Akuakultur, 4(2), 213–219. https://doi.org/http://dx.doi.org/10.15578/jra.4.2.2009.213-219
Chan, M. T. T., Muttray, A., Sakhrani, D., Woodward, K., Kim, J.-H., Christensen, K. A., Koop, B. F., & Devlin, R. H. (2021). Sexually Dimorphic Growth Stimulation in a Strain of Growth Hormone Transgenic Coho Salmon (Oncorhynchus kisutch). Marine Biotechnology, 23, 140–148. https://doi.org/https://doi.org/10.1007/s10126-020-10012-5
Chen, T. T., Agellon, L. B., Lin, C. M., Tsai, H. J., Zhang, P., González-Villasénor, L. I., & Powers, D. A. (1989). Evolutionary implications of two rainbow trout growth hormone genes. Fish Physiology and Biochemistry, 7(1–6), 381–385. https://doi.org/10.1007/BF00004732
Dewi, R. R. S. P. S., Alimuddin, Sudrajat, A. O., & Sumantadinata, K. (2012). PhGH gene transfer effectivity and expression on stripped catfish (Pangasianodon hypophthalmus). Jurnal Riset Akuakultur, 7(2), 171–180. https://doi.org/10.15578/jra.7.2.2012.171-180
Dewi, R. R. S. P. S., Sudrajat, A. O., Alimuddin, & Sumantadinata, K. (2009). Identification of growth hormone gene of Pangasionodon hypophthalmus. Indonesian Aquaculture Journal, 4(1), 9–17. https://doi.org/10.15578/iaj.4.1.2009.9-17
Faridah, N. (2012). Introduction and expression of growth hormone gene on transgenic common carp. IPB University. Bogor. 58 pp.
Hanum, G. R. (2017). Biokimia Dasar (S. B. Sartika (ed.); 1st ed.). UMSIDA Press. Sidoarjo. 154 pp.
Hayuningtyas, E. P., & Kusrini, E. (2016). Performance of wild betta fish (Betta imbellis) growth treated with recombinant growth hormone (rEIGH) through immersion and natural feed food. Media Akuakultur, 11(13), 87–95. https://doi.org/http://dx.doi.org/10.15578/ma.11.2.2016.87-95
Hseu, J., Huang, W., & Chu, Y. (2007). What causes cannibalization‐associated suffocation in cultured brown‐marbled grouper, Epinephelus fuscoguttatus (Forsskål, 1775)? Aquaculture Research, 38(10), 1056–1060. https://doi.org/10.1111/j.1365-2109.2007.01762.x
Kamenskaya, D. N., & Brykov, V. A. (2020). Fish Growth Hormone Genes: Structure and Divergence. Russian Journal of Marine Biology, 46(4), 233–242. https://doi.org/10.1134/S1063074020040033
Kurdianto, Alimuddin, Faridah, N., Yoshizaki, G., Nuryati, S., & Setiawati, M. (2016). Growth, Survival, and Body Composition of Transgenic Common Carp Cyprinus carpio 3rd Generation Expressing Tilapia Growth Hormone cDNA. HAYATI Journal of Biosciences, 23(3), 150–154. https://doi.org/10.1016/j.hjb.2016.12.002
Kusrini, E., Alimuddin, Junior, M. Z., & Soelistyowati, D. T. (2018). Foreign growth hormone gene transmission and expression in F1 Transgenik Betta fish (Betta imbellis). Pakistan Journal of Biotechnology, 15(1), 1–9. http://www.elsevier.com/locate/scp
Kusrini, E., Priyadi, A., & Prasetio, A. B. (2015). Business challenge tiger Catfish (Pseudoplatystoma fasciatum) through controlled spawning technology. Media Akuakultur Vol., 29(2), 79–83. https://doi.org/http://dx.doi.org/10.15578/ma.10.2.2015.79-83
Lemaire, C., Warit, S., & Panyim, S. (1994). Giant catfish Pangasianodon gigas growth hormone-encoding cDNA: cloning and sequencing by one-sided polymerase chain reaction. Gene, 149(2), 271–276. https://doi.org/10.1016/0378-1119(94)90160-0
Lesmana, I. (2010). Production and bioactivity of growth hormone recombinant protein from three types of cultured fish. IPB University. Bogor. 62 pp. http://repository.ipb.ac.id/handle/123456789/57531
Moriyama, S., Ayson, F. G., & Kawauchi, H. (2000). Growth regulation by insulin-like growth factor-i in fish. Bioscience, Biotechnology and Biochemistry, 64(8), 1553–1562. https://doi.org/10.1271/bbb.64.1553
Nugroho, E., Alimuddin, Kristanto, A. H., & Carman, O. (2016). Cloning of β-actin promoter from giant gouramy (Osphronemus gouramy). Jurnal Riset Akuakultur, 4(1), 23–31. https://doi.org/10.15578/jra.4.1.2009.23-31
Nuñez, J., Dugué, R., Arana, N. C., Duponchelle, F., Renno, J. F., Raynaud, T., Hubert, N., & Legendre, M. (2008). Induced breeding and larval rearing of Surubí, Pseudoplatystoma fasciatum (Linnaeus, 1766), from the Bolivian Amazon. Aquaculture Research, 39(7), 764–776. https://doi.org/10.1111/j.1365-2109.2008.01928.x
Permana, A., Alimuddin, Hadie, W., Priyadi, A., & Ginanjar, R. (2018). The effects of using recombinant growth hormone on the growth of Clown Loach (Chromobotia macracanthus) juveniles with different methods of administration. Jurnal Riset Akuakultur, 13(2), 123–130. https://doi.org/10.15578/jra.13.2.2018.123-130
Pinheiro, J. S., Wolff, J. L. C., Araújo, R. de C., & Hilsdorf, A. W. S. (2008). Molecular cloning and sequence analysis of growth hormone cDNA of Neotropical freshwater fish Pacu (Piaractus mesopotamicus). Genetics and Molecular Biology, 31(1 SUPPL. 1), 381–384. https://doi.org/10.1590/s1415-47572008000200037
Safir, M., Alimuddin, Setiawati, M., Junior, M. Z., & Suprayudi, M. A. (2017). Growth Performance of Nile tilapia Immersed in 17α-methyltestosterone and rElGH, and Fed a Diet Enriched with rElGH. Omni-Akuatika, 13(2), 57–64. https://doi.org/10.20884/1.oa.2017.13.2.288
Sembiring, A. A., Tarsim, & Utomo, D. S. C. (2018). The use of recombinant growth hormone (rGH) in stimulating the growth of Black Ghost fish larvae (Apteronotus albifrons). Jurnal Sains Teknologi Akuakultur, 2(1), 51–56. http://digilib.unila.ac.id/30072/
Setyawan, P. K. F., Rejeki, S., & Nugroho, R. A. (2014). The effect of giving recombinant growth hormone (rGH) through immersion methods with different doses on survival and growth of Larasati tilapia larvae (Oreochromis niloticus). Journal of Aquaculture Management and Technology, 3(2), 69–76. https://ejournal3.undip.ac.id/index.php/jamt/article/view/5061
Slater, E. P., Baxter, J. D., & Eberhardt, N. L. (1986). Evolution of the growth hormone gene family. Integrative and Comparative Biology, 26(4), 939–949. https://doi.org/10.1093/icb/26.4.939
Syarifudin, M., Alimuddin, Widyastuti, U., & Aliah, R. S. (2007). cDNA encoding growth hormone from humpbac grouper (Cromileptes altivelis). Biotropia, 14(2), 1–8. https://doi.org/https://doi.org/10.11598/btb.2007.14.2.14
Tang, X., Ye, X., Wu, Q., Tang, Y., & Zhang, D. (2021). Molecular characterization and expression analysis of a novel C-type lectin ( CTL ) gene in yellow catfish Pelteobagrus fulvidraco. Aquaculture Reports, 20(February), 100640. https://doi.org/10.1016/j.aqrep.2021.100640
Triwinarso, W. H., Basuki, F., & Yuniarti, T. (2014). Effect of Recombinant of Growth Hormone (rGH) through Immersion Method with Different Time on Growth and Survival Catfish Varieties Sangkuriang. Journal of Aquaculture Management and Technology, 3(4), 265–272. http://ejournal-s1.undip.ac.id/index.php/jfpik
Wood, A. W., Duan, C., & Bern, H. A. (2005). Insulin-like growth factor signaling in fish. International Review of Cytology, 243, 215–285. https://doi.org/10.1016/S0074-7696(05)43004-1
DOI: http://dx.doi.org/10.15578/iaj.17.2.2022.107-119
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indonesian Aquaculture Journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
_25.jpg)
