EFFICACY OF GP-11 KHV DNA VACCINE IN Cyprinus carpio koi

Sekar Ayu Chairunnisa, Sri Nuryati, Alimuddin Alimuddin, Sri Murtini, Ayi Santika, Dwi Hany Yanti

Abstract


Koi herpesvirus (KHV) is one of the major pathogen for koi and common carp which cause high mortality and economic losses for the farmer. The purpose of this study was to determine the efficacy of glycoprotein-11 (GP-11) KHV DNA vaccine and compared to GP-25 KHV DNA vaccine. The vaccine in the form of naked DNA plasmidwas delivered by intramuscularly injection to the 3-month-old koi. The fish were divided into six groups, i.e. unvaccinated group (negative control C- and  positive control C+), and vaccinated group (2.5 μg/100 μl of GP-11 (group 1), 7.5 μg/100 μl of GP-11 (group 2), 12.5 μg/100 μl of GP-11 (group 3), and 12.5 μg/100 μl of GP-25 (group 4)). At day 42 post vaccination, all fish of each groups were challenged by injecting KHV titre 10-3 FID50. Number of dead fish was counted everyday after the challenge until 30 days. The results showed that vaccinated fish were had survival rate of 83.33-93.33% (group 2, 3 and 4). It’s show that GP-11 KHV DNA vaccine has high efficacy. As conclusion, GP-11 DNA vaccine could be an alternative DNA vaccine for preventing KHV infection.

Keywords


Cyprinus carpio koi; DNA vaccine; injection; koi herpesvirus

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References


Alimuddin, Lola, I.P., Fahrudin, M.H.A., Chairul, M., Carman, O., & Sumantadinata K. (2009). Aktivitas promoter β-aktin ikan medaka Jepang (Oryzia slatipes) pada ikan mas (Cyprinus carpio). Jurnal Natur Indonesia, 11, 70-77.

Aoki, T., Hirono, I., Kurokawa, K., Fukuda, H., Nahary, R., Eldar, A., Davison, A.J., Waltzek, T.B., Bercovier, H., & Hedrick, R.P. (2007). Genome sequences of three koi herpesvirus isolates representing the expanding distribution of an emerging disease threatening koi and common carp worldwide. Journal of Virology, 81, 5058-5066.

Burton, D.R. (2002). Antibodies, viruses and vaccines. Nature Reviews, 2, 706-713.

Clauss, T.M., Dove, A.D.M., & Arnold, J.E. (2008). Hematologic disorders of fish. Vet ClinExotAnim, 11, 445-462.

Davison, A.,J. (2010). Herpesvirus systematics. Veterinary Microbiology, 143, 52-69.

Dimmock, N.J. (1995). Update on the neutralization of animal viruses. Rev Med Virol, 5, 165–179.

Donnelly, J.J., Jeffrey, B.U., John, W.S., & Margaret, A.L. (1997). DNA Vaccines. Annu Rev Immunol, 15, 617-648.

Hedrick, R.P., Gilad, O., Yun, S., & Spangenberg, J.V. (2000). A herpesvirus associated with mass mortality of juvenile and adult koi, a strain of common carp. J Aquat Animal Health, 12, 44-57.

Kollner, B., Wasserab, B., Kotterba, G., & Fischer, U. (2002). Evaluation of immune functions of rainbow trout (Onchorinchus mykiss)-how can environmental influences be detected? J Toxicology Letters, 131, 83-95.

Kresno, S.B. (2001). Imunologi: Diagnosis dan Prosedur Laboratorium, Edisi Keempat (p.341). Balai Penerbit Fakultas Kedokteran Universitas Indonesia. Jakarta.

Kurath, G. (2008). Biotechnology and DNA vaccines for aquatic animals. Revue Scientifique et Technique. International Office of Epizootics, 27(1), 175-196.

Lorenzen, N., & LaPatra, S.E. (2005). DNA vaccines for aquacultured fish. Revue Scientifique et Technique. International Office of Epizootics, 24, 201-213.

Nuryati, S., Alimuddin, Sukenda, Soejoedono, R.D., Santika, A., Pasaribu, F.H., & Sumantadinata, K. (2010a). Construction of a DNA Vaccine using glycoprotein gene and its expression towards increasing survival rate of KHV-Infected common carp (Cyprinus carpio). Jurnal Natur Indonesia, 13, 47-52.

Nuryati, S., Maswan, N.A., Almuddin, Sukenda, Sumantadinata, K., Pasaribu, F.H., Soejoedono, R.D., & Santika, A. (2010b). Hematology of common carp following DNA vaccination and koi herpesvirus challenge test. Jurnal Akuakultur Indonesia, 9(1), 9-15.

Perelberg, A., Smirnov, M., Hutoran, M., Diaman, A., Bejerano, Y., & Kotler, M. (2003). Epidemiological description of a new viral disease afflicting cultured Cyprinuscarpioin Israel. Israeli Journal Aquaculture-Bamidgeh, 55, 5-12.

Pikarsky, E., Ronen, A., Abramowitz, J., Lovali-Sivan, B., Hutoran, M., Saphira, Y., Steinitz, M., Perelberg, A., Soffer, D., & Kotler, M. (2004). Pathogenesis of acute viral disease induce in fish by carp interstitial nephritis and gill necrosis virus. Journal of Virology, 78(17), 9544-9551.

Rawat, M., Singh, D., Saraf, S., & Saraf, S. (2007). An overview of biochemical aspects of DNA vaccine. Asian Journal of Biochemistry, 2(4), 208-223.

Taukhid, Sumiati, T., & Koesharyani, L. (2005). Pengaruh suhu air dan total bahan organic terlarut terhadap patogenisitas koi herpesvirus pada ikan mas (Cyprinus carpio). Serial Bunga Rampai: Strategi Pengelolaan dan Pengendalian Penyakit KHV, suatu upaya pemecahan dalam pembudidayaan ikan air tawar. Pusat Riset Perikanan Budidaya, Badan Riset Kelautan dan Perikanan, Departemen Kelautan dan Perikanan.

Tizard, I. (1988). An introduction to Veterinary Immunology. 2nd edition. Wb. Philadelphia: Sanders Company.

Tonheim, T.C., BØgwald, J., & Dalmo, R.A. (2008). What happens to the DNA vaccine in fish? A review of current knowledge. Fish & Shellfish Immunology, 25, 1-18

Triyanto, Kamiso, H.N., Isnansetyo, A., & Murwantoko. (1997). Pembuatan antigen murni untuk memproduksi polivalen antibody dan vaksin Aeromonas hydrophilla. Laporan penelitian hibah bersaing V/1 perguruan tinggi tahun anggaran 1996/1997. Fakultas Pertanian UGM. Yogyakarta.

Yazawa, R., Hirono, I., & Aoki, T. (2005). Characterization of promoter activities of four different Japanese flounder promoters in transgenic zebrafish. Marine Biotechnology, 7, 625-633.

Yuasa, K., Ito, T., & Sano, M. (2008). Effect of water temperature on mortality and virus shedding in carp experimentally infected with koi herpesvirus. Fish Pathol, 43, 83–85.

Zhou, J., Xue, J., Wang, Q., Zhu xia., Li, X., Lv, W., & Zhang, D. (2014). Vaccination of pasmid DNA ORF 81 gene of CJ strains of KHV provides protections to immunized carp. In Vitro Cell DevBiol—Animal, 50, 489–495.




DOI: http://dx.doi.org/10.15578/iaj.11.1.2016.31-39



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