92 Citations
COMPARATIVE ASSESSMENT OF THE EFFECT OF GIBBERELLIC AND SALICYLIC ACIDS ON THE GROWTH AND BIOCHEMICAL PARAMETERS OF Phaeodactylum tricornutum
Abstract
The research of the effect of gibberellic (0.4 - 3.8x 10-8 mol.L-1) and salicylic (0.4 - 3.8 x 10-5 mol.L-1) acids, in a wide range of their concentrations, on the growth indicators and biochemical composition of the cumulative culture of the microalgae Phaeodactylum tricornutum was carried out. It was determined that salicylic acid in a concentration of 0.4 x 10-5 mol stimulated cell growth by 184.6%, and gibberellic acid at a concentration of 0.39 x 10-8 mol by 181%, compared to the control. The effect of gibberellic acid during the experiment was expressed in the inhibition of protein accumulation in the culture, compared with the control. The use of salicylic acid led to a greater accumulation of protein in the culture than when using gibberellic acid. It was shown that salicylic acid had a positive effect on the accumulation of carbohydrates on day 9 and gibberellic acid on day 14 of culture. Gibberellic acid had no effect on the accumulation of lipids in the culture of microalgae. Under the action of salicylic acid for 14 days of cultivation, the lipid content increased by 18.5%, compared with the control. There were no quantitative differences in the content of chlorophyll when using two phytohormones. In this study, the optimal concentrations of gibberellic and salicylic acids for linear growth rate and the highest production of protein and carbohydrates for Phaeodactylum tricornutum were determined. Position, depending on the stage of microalgae growth, is noted.
Keywords
Full Text:
PDFReferences
Aminot A., & Ray F. (2000). Standard Procedure for the Determination of Chlorophyll a by Spectroscopic Methods (p. 17) Copenhagen, Denmark: International Council for the Exploration of the Sea (ICES Techniques in Marine Environmental Sciences).
Bai, X., Song, H, Lavoie, M., Zhu, K., Su, Y., Ye, H., … Qian, H. (2016). Proteomic analyses bring new insights into the effect of a dark stress on lipid biosynthesis in Phaeodactylum tricornutum. Scientific Reports, 6, 254–264.
Basra, A. (2000). Plant Growth Regulators in Agriculture and Horticulture: Their Role and Commercial Uses. USA: CRC Press.
Batista, A. P., Niccolai, F., Bursic, I., Sousa, I., Raymundo, A., Rodolû, L., … Tredici, M. R. (2019). Microalgae as Functional Ingredients in Savory Food Products: Application to Wheat Crackers. Foods, 8, 611-635.
Burch, A. R., Yothers, C. W., Salemi, M. R., Phinney, B. S., Pandey, P., & Franz, A. R. (2021). Quantitative label-free proteomics and biochemical analysis of Phaeodactylum tricornutum cultivation on dairy manure wastewater. Journal of Applied Phycology, 33, 2105 – 2121.
Butselaar, V. T., & Ackerveken, G. (2020). Salicylic acid steers the growth-immunity trade off. Trends in Plant Science, 25, 566–576.
Caporgno, M. P., & Mathys, A. (2018). Trends in Microalgae Incorporation Into Innovative Food Products With Potential Health Benefits. Frontier in Nutrition, 5, https://doi.org/10.3389/fnut.2018.00058
Carneiro, M., Pojo, V., Malcata, F. X., & Otero, A. (2019). Lipid accumulation in selected Tetraselmis strains. Journal of Applied Phycology, 31, 2845–2853.
Chu, J., Li, Y., Cui, Y., & Qin, S. (2019). The influences of phytohormones on triacylglycerol accumulation in an oleaginous marine diatom Phaeodactylum tricornutum. Journal of Applied Phycology, 31, 1009–1019.
Dragone, G., Bruno, D., Fernandes, B. D., Abreu, A. P., Vicente, A. A., & Teixeira, J. A. (2011). Nutrient limitation as a strategy for increasing starch accumulation in microalgae. Applied Energy, 88, 3331–3335.
Du, H., Ahmed, F., Lin, B., Li, Z., Huang, Y., Sun, G., … Gao, Z. (2017). The Effects of Plant Growth Regulators on Cell Growth, Protein, Carotenoid, PUFAs and Lipid Production of Chlorella pyrenoidosa ZF Strain. Energies, 10, 1696 – 1719.
Hamilton, M. L., Powers, S., Napier, J. A., & Sayanova, O. (2016). Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornutum. Marine drugs, 14, 53-63.
Hedden, P., & Sponsel, V. A. (2015). Century of Gibberellin Research. Journal of Plant Growth Regulation, 34, 740–760.
Helm, M. M., Bourne, N., & Lovatelli, A. (2004). Hatchery culture of bivalves, a practical manual. FAO Fisheries Technical Paper 471. Food and Agriculture Organization of the in the laboratory. Marine Biology, 15, 350-355.
Herbert, D., Phipps, P. J., & Strange, R. E. (1971). Chapter III Chemical Analysis of Microbial Cells. Methods in Microbiology, 209–344.
Johnson, K.R., Ellis, G., & Toothill, C. (1977). The sulfophosphovanilin reaction for serum lipids: a reappraisal. Clinical Chemistry, 23, 1669–1678.
Laurens, L. M. L., Dempster, T. A., Jones, H. D. T., Wolfrum, E. J., Van Wychen, S., McAllister, J. S. P., … Gloe L. M. (2012). Algal biomass constituent analysis: Method uncertainties and investigation of the underlying measuring chemistries. Analytical Chemistry, 84(4), 1879–1887.
Lowry, O. H., Rosenbrougt, N. J., Farr, A. L., & Randal, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265–276.
Lu, Y., & Xu, J. (2015). Phytohormones in microalgae: A new opportunity for microalgal biotechnology. Trends in Plant Science, 20, 273–282.
Miceli, A., Romano, C., Moncada, A., Piazza, G., Torta, L., D’Ann, F., & Vetran, F. (2019). Yield and quality of mini-watermelon as aûected by grafting and mycorrhizal inoculum. Journal of Agricultural Science and Technology, 18, 505–516.
Mishra, A. K., & Baek, K-H. (2021). Salicylic Acid Biosynthesis and Metabolism: A Divergent Pathway for Plants and Bacteria. Biomolecules, 11, 705-717.
Rasdi, N. W., & Qin, J. G. (2015). Effect of NP: Ratio on growth and chemical composition of Nannochloropsis oculata and Tisochrysis lutea. Journal of Applied Physiology, 27, 2221–230.
Rodas-Junco, B. A., Nic-Can, G. I., Munoz-Sanchez, A., & Hernandez-Sotomayor, S. M. T. (2020). Phospholipid Signaling Is a Component of the Salicylic Acid Response in Plant Cell Suspension Cultures. International Journal of Molecular Sciences, 21, 52 – 85.
Shahzad, K., Hussain, S., Arfan, M., Hussain, S., Waraich, E. A., Zamir, S., … Hano C. (2021). Exogenously Applied Gibberellic Acid Enhances Growth and Salinity Stress Tolerance of Maize through Modulating the Morpho-Physiological, Biochemical and Molecular Attributes. Biomolecules, 11, 1005 – 1022.
Sharma, N., Fleurent, G., Awwad, F., Cheng, M., Meddeb-Mouelhi, F., Budge, S. M., & Desgagne-Penix, I. (2020). Red Light Variation an Eûective Alternative to Regulate Biomass and Lipid Proûles in Phaeodactylum tricornutum. Applied. Sciences, 10, 25 – 31.
Tarakhovskaya, E. R., Maslov, Y. I., & Shishova, M. F. (2007). Phytohormones in algae. Russian Journal of Plant Physiology, 54,163–170.
Van Hung, P. (2016). Phenolic compounds of cereals and their antioxidant capacity. Critical Reviews in Food Science and Nutrition, 56, 209–344.
Wallis, C. M., & Galarneau, E. R-A. (2020). Phenolic Compound induction in plant-microbe and plant-insect interactions: A meta-analysis. Frontiers inPlant Science, 11, 2034 – 2047.
Xu, J., Fan, X., Li, X., liu, G., Zhang, Z., Zhu, Y., … Qian, H. (2017). Effect of salicylic acid on fatty acid accumulation in Phaeodactylum tricornutum during stationary growth phase. Journal of Applied Phycology, 29, 2801–2810.
Yang L., Chen J., Qin S., Zeng., Jiang Y., Hu L., … Wang J. (2008) Growth and lipid accumulation by different nutrients in the microalga Chlamydomonas reinhardtii. Biotechnol. Biofuels, 11, 40 - 51.
Zhang, H., Yin, W., Ma, D., Liu, X., Xu, K., & Liu, J. (2021). Phytohormone supplementation significantly increases fatty acid content of Phaeodactylum tricornutum in two-phase culture. Journal of Applied Phycology, 33, 13–23.
DOI: http://dx.doi.org/10.15578/iaj.18.1.2023.37-44
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indonesian Aquaculture Journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
_25.jpg)
