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INDUCTION OF GONADAL MATURATION OF POND CULTURED MALE TIGER SHRIMP, Penaeus monodon WITH DIFFERENT DOSAGES OF GONADOTROPIN RELEASING HORMONE ANALOGUE AGAINST EYE STALK ABLATION
Abstract
Very low naturally mating rate of pond-reared tiger shrimp broodstock is probably due to the slow maturation of the male stock. The aim of this study was to evaluate the salmon gonadotrophin releasing hormone analoque (sGnRHa) in stimulating the gonadal maturation of male stock of pond-reared tiger shrimp. The treatments were three dosages of sGnRHa at 0.1 (OV-1), 0.2 (OV-2), and 0.3 (OV-3) mL/kg of shrimp weight and control was eye stalk ablation (AB). The sGnRHa was administered via injection three times with one week interval. Male stocks with average initial body weight of 82.1 g were randomly distributed into four of 10 m3 concrete tanks, 26 males for each tank. Variables observed were performances of spermatophores and profiles of amino acid and fatty acid of muscle of the male stocks. After induction, number of male maturing indicated by spermatophores releasing from terminal ampullas was higher in shrimp induced with OV-1 (80.8%) compared to control which was only 46.1%. Furthermore, shrimp treated OV-2 had the highest spermatophore weight of 0.16 g compared to control (0.11 g) and other two groups. Amino acid profiles improved as the dose of sGnRHa increased up to 0.2 mL/kg from 61.23% for ablated male becoming 71.27% for OV-2. Total fatty acid also tended to improve by increasing the dose of hormone injection, however, the ablated male had higher total fatty acid content than that of OV-1. The present finding demonstrated that the dose of sGnRHa to stimulate the gonadal maturation of pond-reared male tiger shrimp could be applied at range between 0.1-0.2 mL/kg of shrimp weight.
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Alfaro, J., Zuniga, G. & Komen, J. 2004. Induction of ovarian maturation and spawning by combined treatment of serotonin and a dopamine antagonist, spiperone in Litopenaeus stylirostris and Litopenaeus vannamei. Aquaculture, 236: 511-522.
AOAC International. 1999. Official Methods of Analysis, 16th edn. Association of Official Analytical Chemists International, Gaithersberg, Maryland, USA. 1141 pp.
Bray, W.A. & Lawrence, A.L. 1992. Reproduction on Penaeus species in captivity. In: Fast A.W. and Lester L.J. (Eds). Marine Shrimp Culture: Principles And Practices. Developments In Aquaculture And Fisheries Science (pp. 93–170). Elsevier, The Netherlands.
Cejko, B.I., Targoriska, K., Kowalski, R.K., Zarski, D., Sarosiek, B., Kucharczyk, D. & Glogowski.J. 2012. The effectiveness of hormonal preparation (Ovopel, Ovaprim, LHRHa, hCG and CPE) in stimulating spermiation in dace Leuciscus leuciscus (L). Journal of Applied Ichthyology, 28: 873-877.
Cejko, B.I. & Krejszeff, S. 2016. Sperm characteristics of chub Leuciscus cephalus (L.) collected in artificial condition after Ovopel and Ovaprim treatment. Aquaculture Research, 47: 847-856.
Coman, G.J., Crocos, P.J., Arnold, S.J., Keys, S.J., Murphy, B. & Preston, N.P. 2007. Growth, survival and reproductive performance of domesticated Australian stocks of the giant tiger prawn, Penaeus monodon reared in tanks and raceways. Journal of World of Aquaculture Society, 36: 464-479.
Diwan, A.D. & Joseph, S. 2009. Cryopreservation of gametes. In Diwan, A.D., Joseph.S., Ayyappan, S. (Ed.) Physiology of reproduction, breeding and culture of tiger shrimp, Penaeus monodon (Fabricius) (pp.145-169). First edition. Narendra Publishing House. Delhi, India.
Genz, J., McDougall, C.A., Kheeto, S., Arcinas, L., Burnett, D. & Anderson, W.G. 2014. Induced spawning of wild-caught adult lake sturgeon: assessment of hormonal and stress responses, gamete quality and survival. Journal of Applied Ichthyology, 30: 1565-1577.
Guzman, J.M., Ramos, J., Mylonas, C.C. & Mananos, E. 2011. Comparative effects of human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone agonist (GnRHa) treatments on the stimulation of male Senegalese sole (Solea senegalensis) reproduction. Aquaculture, 316: 121-128
Huang H, Ye H, Li S. & Wang, G. 2008. Immunocytological evidence for the presence of vertebrate FSH and LH-like substances in the brain and thoracic ganglion of the swimming crab, Portunus trituberculatus. Progress in Natural Science, 18:1453-1457
Jiang, S-H., Huang, J-H, Zhou, F-L., Chen, X., Yang, Q-B., Wen, W-G. & Ma, Z-M. 2009. Observation of reproductive development and maturation of male Penaeus monodon reared in tidal and earthen pond. Aquaculture, 292: 121-128
Jamroz, M., Kucharczyk, D., Hakuc-Blazowska, A., Krejszeff, S., Kujawa, R., Kupren, K., Kwiatkowski, M., Targonska, K., Zarski, D., Cejko, B. & Glogowski, J. 2008. Comparing the effectiveness of ovopel. Ovaprim, and LH-RH analogue used in the controlled reproduction of Ide, Leuciscus idus (L.). Archives of Polish Fisheries, Volume 16 Issue 4: 363-370.
Kagawa, H., Fujie, N., Imaizumi, H., Masuda, Y., Oda, K., Adachi, J., Nishi, A., Hashimoto, H., Teruya, K. & Kaji, S. 2013. Using osmotic pumps to deliver hormones to induce sexual maturation of female Japanese eels, Anguilla japonica. Aquaculture, 388-391: 30-34
Komatsu, T., Bhandari, R.K., Kobayashi, Y., Nakamura, S. & Nakamura, M. 2006. GnRHa-accelerated spermatogenesis in the testes of underyearling golden rabbitfish, Siganus guttatus (Bloch). Aquaculture, 257: 558-565.
Krol, J., Kowalski, R.K., Hilwa, P., Dietrich, G.J., Stabinski, R. & Ciereszko, A. 2009. The effects of commercial preparation containing two different GnRH analogues and dopamine antagonists on spermiation and sperm characteristics in the European smelt Osmerus epertanus (L.). Aquaculture, 286: 328-333.
Laining, A., Usman & Rachmansyah. 2014a. The use of seaworm meal in maturation diet as partial substitution of fresh-diet for pond reared tiger shrimp broodstock, Penaeus monodon. Indonesian Aquaculture Journal, Volume 9 Number 2: 123-132.
Laining, A., Usman, Muslimin & Neltje N.P. 2014b. Performansi Pertumbuhan dan Reproduksi Udang Windu Asal Tambak yang Diberi Kombinasi Pakan yang Berbeda. Jurnal Riset Akuakultur, Volume 9 Nomor 1: 67-77.
Laining, A., Lante, S. & Usman. 2014c. Inseminasi buatan sebagai upaya peningkatan fertilitas dan daya tetas telur induk udang windu, Penaeus monodon. Buku abstrak Simposium Nasional Bioteknologi Akuakultur V, 11 September 2014, IPB Internasional Center, Bogor.
Laining, A., Lante, S. & Usman. 2015a. Induksi pematangan gonad dan peningkatan tingkat pembuahan telur induk udang windu, Penaeus monodon melalui rangsangan hormonal tanpa ablasi mata. Jurnal Riset Akuakultur, Vol.10 No.1: 61-68
Laining, A., Lante, S. & Parenrengi, A. 2015b. Gonadosomatic index and profile of fillet fatty acid of rabbitfish, Siganus guttatus broodstock fed carotenoid supplemented diets. Proceeding of International Conference on Aquaculture Indonesia, Jakarta, 28-31 Oct 2015.
Primavera, J.H. 1978. Induced maturation and spawning in five-month-old Penaeus monodon Fabricius by eyestalk ablation. Aquaculture, Volume 13 Issue 4: 355-359.
Sherwood, N. M. & Adams, B.A. 2005. Gonadotropin-releasing hormone in fish: evlution, expression and regulation of the GnRH gene. In Melamed, P., Sherwood, N. (Ed.) Hormones and their receptors in fish reproduction. Molecular aspects of fish and marine biology Vol.4 (pp. 1-39). World Scientific Publishing Co.Pte.Ltd. Singapore.
Menasveta, P., Sangpradub, S., Piyatiratitivorakul, S. & Fast, A.W. 1994. Effect of broodstock size and source on ovarian maturation and spawning of Penaeus monodon Fabricus from the Gulf of Thailand. Journal of World Aquaculture Society, 25: 41-49.
Pongtippatee, P., Vanichhviriyakit, R., Chavadej, J., Plodpai, P., Pratoomchart, B., Sobhon B. & Withyachumnarnkul, B. 2007. Acrosome reaction in the sperm of the black tiger shrimp, Penaeus monodon (decapoda, Penaidae). Aquaculture Research, 38: 1635-1644.
Primavera, J.H. 1978. Induced maturation and spawning in 5 month old Penaeus monodon Fabricius by eye stalk ablation. Aquaculture, 13: 355-359.
Sellars, M.J., Keys, S.K., Cowley, J.A., McCulloch, R. & Preston, N. P. 2006. Effect of rearing environment on Mourilyan Virus load in Penaeus japonicus during maturation in tank systems. Aquaculture, 252: 240-245.
Tinikul, Y., Poljaroen, J., Tinikul, R., Anuracpreeda, P., Chotwiwatthanakun, C., Senin, N., Poomtong, T., Hanna, P.J. & Sobhon, P. 2014. Effects of gonadotropin-releasing hormones and dopamine on ovarian maturation in the Pacific white shrimp, Litopenaeus vannamei, and their presence in the ovary during ovarian development. Aquaculture, 420-421: 79-88.
Wongprasert, K., Asuvapongpatana, S., Poltana, P., Tiensuwan, M. & Withyachumnarnkul, B. 2006. Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp, Penaeus monodon. Aquaculture, 261: 1447-1454.
Zadmajid, V. 2016. Comparative effects of human chorionic gonadotropin (hCG) and OvaprimTM (sGnRHa + domperidone) on the reproductive characteristics of wild-caught male Longspine craper, Capoeta trutta (Heckel, 1843). Aquaculture, 463: 7-15.
Zarski, D., Kucharczyk, D., Targonska, K., Jamroz, M., Krejszeff, S. & Mamcarz, A. 2009. Application of ovopel dan ovaprim and their combinations in controlled reproduction of two reophilic cyprinid fish species. Polish Journal of Natural Sciences, Volume 24 (4): 235-244.
Zhuo, Q., Zhang, Y., Huang, W., Liu, X., Li, Y., Zhu, P., Lu, D. & Lin, H. 2012. Gonadotropin-releasing hormone analogue multiple injention potentially accelerated testicular maturation of male yellow catfish (Pelteobagrus fluvidraco, Richardson) in caprivity. Aquaculture Research, 43: 467-480.
DOI: http://dx.doi.org/10.15578/iaj.11.1.2016.23-30
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