STUDY PROPAGATION OF GSDIV (GROUPER SLEEPY DISEASE IRIDOVIRUS) IN CULTURED GF (GRUNT FIN) CELL

Ketut Mahardika, Indah Mastuti

Abstract


Grouper Sleepy Disease Iridovirus (GSDIV, genus Megalocityvirus) remain unsolved in Indonesian aquaculture. A GSDIV propagating study had been conducted to elaborate the capability of GSDIV replication in GF cell line. GSDIV isolate derived from spleen of diseased grouper fish were infected to GF cells line. GSDIV-infected GF cells were characterized by enlargement cells as CPE (cytophatic effect) which floated in cultured-medium. CPE-GSDIV was positively confirmed by PCR test. GSDIV-viral concentrations were ranging between 102.80-105.80 TCID50/mL. The newly passage GF cells were more sensitive to GSDIV which was shown by the abundance of CPE. GSDIV derived from GF cells were caused 10%-100% mortality of humpback grouper, Cromileptes altivelis.


Keywords


GSDIV; grunt fin cells; CPE; C. altivelis

Full Text:

PDF


DOI: http://dx.doi.org/10.15578/iaj.8.2.2013.121-126


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

View My Stats
p-ISSN: 0215-0883
e-ISSN: 2502-6577


Find in a library with WorldCatCrossref logoSHERPA/RoMEO LogoHasil gambar untuk isjdgoogle scholardoaj