THE EFFECT OF VITAMIN C (L-ASCORBYL MONOPHOSPHATE-Mg) ON THE DEFORMITY PERFORMANCE OF HUMPBACK GROUPER (Cromileptes altivelis) LARVAE

Sophia Lasma Sagala, Suko Ismi, I Nyoman Adiasmara Giri

Abstract


This study was aimed to get a standard protocol for vitamin C application in enrichment of Artemia nauplii and to examine the impact of vitamin C on humpback grouper larval deformity performance. Vitamin C in the form of L-ascorbyl-2-monophosphate-Mg (AMP-Mg) was used for the enrichment and incorporated into Artemia nauplii at a given level of 0-1.5 g/L with enrichment period of 4, 6, and 24 hours for each level. The effect of AMP on larval deformity was tested using the following treatments: unenriched both Artemia nauplii and commercial diet, un-enriched Artemia nauplii and experimental diet containing 2.5 g AMP-Mg/kg diet, enriched Artemia nauplii obtained from the protocol and commercial larval diet. Analysis of vitamin C content in Artemia nauplii was carried out using HPLC and larval deformities were observed using staining method, visual examination, or X-ray depending on larval age. The results showed that the best enrichment method of AMP in Artemia nauplii is achieved at the dose of 0.9 g/L for 6 h. The lowest percentage of abnormalities was found in larvae fed with enriched Artemia nauplii and commercial diets, suggesting that vitamin C has an important role in controlling larval deformities.


Keywords


humpback grouper larvae; vitamin C; deformity

Full Text:

PDF


DOI: http://dx.doi.org/10.15578/iaj.5.1.2010.29-36


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

View My Stats
p-ISSN: 0215-0883
e-ISSN: 2502-6577


Find in a library with WorldCatCrossref logoSHERPA/RoMEO LogoHasil gambar untuk isjdgoogle scholardoaj