Tuty Arisuryanti, Gregorius Altius Pratama, Lukman Hakim, Johan Putra Koentjana, Fitria Kurnia Nazira


Genetic characterization data of kissing gourami are important to understand historical lineage thus enhancing sustainability of the species and to establish regulation for sustainable management of the fish stock in their habitat. However, investigation of genetic characterization of kissing gourami, one of native Indonesian freshwater fishes has poorly understood. Therefore, the aim of this study was to examine genetic characterization of the fish species collected from Ogan River, South Sumatra using partial sequences of two mitochondrial genes, 16S rRNA and COI. The results revealed that for the 621 bp determined in 16S rRNA gene of the samples, five sites were variable, of which one was parsimony informative. Concatenate data revealed three haplotypes with an overall haplotype diversity of 0.833±0.222 and nucleotide diversity of 0.003±0.001. The genetic divergence varied from 0-0.49%. Next, sequence analysis of COI gene exhibited 609 bp which can be translated into 203 amino acids. For the 609 bp sequence determined in the fish samples, three haplotypes were revealed with nine variable sites and two parsimony informatives. Haplotype diversity and nucleotide diversity of the fish samples were 0.833±0.22 and 0.00794±0.0025, respectively. The haplotype divergence between the fish samples was also supported by three nonsynonymous codons. In addition, the genetic divergence varied from 0 % to 1.16 %. The results suggest that genetic variation of the kissing gourami has to be monitored and further studies are needed to compare the same species from different location to know the historical lineage and demography.


Genetic variation; kissing gourami; mt-DNA; Ogan River

Full Text:



Arisuryanti, T. (2016). Molecular genetic and taxonomic studies of the swamp eel (Monopterus albus Zuiew 1793). PhD Thesis, Charles Darwin University, Australia.

Arisuryanti, T., Wei, N.-W.V. & Austin, C. (2016). Molecular evidence for determination cryptic species of Indonesian swamp eel populations using denaturing gradient gel electrophoresis (DGGE). AIP Conference Proceedings, 1744, 020060(1)-020060(6).

Arisuryanti, T., Hasan, R.L., & Koentjana, J.P. (2018). Genetic identification of two mudskipper species (Pisces: Gobiidae) from Bogowonto Lagoon (Yogyakarta, Indonesia) using COI mitochondrial gene as a DNA barcoding marker. AIP Conference Proceedings, 2002, 020068(1)-020068(7).

Belton, B., & Thilsted, S.H. (2014). Fisheries in transition: Food and nutrition security implications for the global South. Global Food Security, 3, 59-66.

Bogard, J.R. Thilsted, S.H., Geoffrey, C.M., Abdul Wahab, M., Hossain, M.A.R., Jacobsen, J., & Stangoulis, J. (2015). Nutrient composition of important fish species in Bangladesh and potential contribution to recommended nutrient intakes. Journal of Food Composition and Analysis, 42, 120-133.

Casey, J., Jardim, E., & Martinsohn, J.T. (2016). The role of genetics in fisheries management under the E.U. common fisheries policy. Journal of Fish Biology, 89, 2755-2767.

Dahruddin, H., Hutama, A., Busson, F., Sauri, S., Hanner, R., Keith, P., Hadiaty, R. & Hubert, N. (2017). Revisiting the ichthyodiversity of Java and Bali through DNA barcodes: taxonomic coverage, identification accuracy, cryptic diversity and identification of exotic species. Molecular Ecology Resources, 17(2), 288-299.

Ilmi, W., & Arisuryanti, T. (2018). Composition of mitochondrial DNA 16S nucleotide of dwarf snakehead (Channa gachua Hamilton, 1822) from Keji River, Magelang, Central Java. Journal of Tropical Biodiversity and Biotechnology, 3(2), 57-61.

Ji, W., Zhang, G., Ran, W., Gardner, J.P.A., Wei, K., Wang, W., & Zou, G. (2014). Genetic diversity of and differentiation among five populations of blunt snout bream (Megalobrama amblycephala) revealed by SRAP markers: Implications for conservation and management. PLoS One, 9(9), e108967.

Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111-120.

Kottelat, M. (2013). The fishes of the inland waters of Southeast Asia: A Catalogue and core bibliography of the fishes known to occur in freshwaters, mangroves and estuaries. The Raffles Bulletin of Zoology, Supplement No. 27, 1-663.

Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: molecular evolutionary genetic analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870-1874.

Li, C.Y., Li, J.T., Xiao, G.Y., Hou, G.Y., & Sun, X.W. (2014). Complete mitochondrial genome of the kissing gourami, Helostoma temminckii. Mitochondrial DNA, 26(6), 1-2.

Librado, P., & Rozas, J. (2009). DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25(11), 1451-1452.

Lind, C.E., Brummett, R.E., & Ponzoni, R.W. (2016). Exploitation and conservation of fish genetic resources in Africa: issues and priorities for aquaculture development and research. Reviews in Aquaculture, 4(3), 125-141.

Maddison, W.P., & Maddison, D.R. (2018). Mesquite: A modular system for evolutionary analysis. Version 3.51

Mahmood, H.M., & Ahmad, S.M. (2017). Advances in molecular markers and their applications in aquaculture and fisheries. Genetics of Aquatic Organisms, 1, 27-41.

Morin, P.A., Martien, K.K., Archer, F.I., Cipriano, F., Steel, D., Jackson, J., & Taylor, B.L. (2010). Applied conservation genetics and the need for quality control and reporting of genetic data used in fisheries and wildlife management. Journal of Heredity, 101(1), 1-10.

Palumbi, S.R. (1996). Nucleid acids II : The Polymerase Chain Reaction. in: Molecular Systematic, (Eds.) D.M. Hillis, C. Moritz, B.K. Mable, Sinauer Associates. Sunderland. Massachusetts, pp. 205-247.

Peninal, S., Subramanian, J., Elavarasi, A., & Kalaiselvam, M. (2017). Genetic identification of marine eels through DNA barcoding from Parangipettai coastal waters. Genomics Data, 11, 81-84.

Sulaiman, Z.H., & Daud, H.K.H. (2002). Pond aquaculture of kissing gouramis Helostoma temminckii (Pisces: Helostomatidae) in Bukit Udal, Tutong: a preliminary investigation. Bruneiana: Anthology of Science Articles, 3, 34–41.

Syaifudin, M., Jubaedah, D., Muslim, M., & Daryani, A. (2017). DNA authentication of Asian Red Catfish Hemibragus nemurus from Musi and Penukal River, South Sumatra Indonesia. Genetics of Aquatic Organisms, 1, 43-48.

Tan, H. H., & Ng, P. K. L. (2005). The labyrinth fishes (Teleostei: Anabantoidei, Channoidei) of Sumatra, Indonesia. Raffles Bulletin of Zoology Supplement, 13, 115-138.

Vidthayanon, C. (2012). Helostoma temminckii. The IUCN Red List of Threatened Species 2012: e.T181326A1722150. Downloaded on 08 October 2018.

Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R., & Hebert, P.D. (2005). DNA barcoding Australia's fish species. Philos Trans R Soc Lond B Biol Sci, 360(1462), 1847-1857.

Wibowo, A., Sloterdijk, H., & Ulrich, P. (2015). Identifying Sumatran peat swamp fish larvae through DNA barcoding, evidence of complete life history pattern. Procedia Chemistry, 14, 76-84.

Zemlak, T.S., Ward, R.D., Connell, A.D., Holmes, B.H., & Hebert, P.D. (2009). DNA barcoding reveals overlooked marine fishes. Molecular Ecolology Resources, 9 Suppl s1, 237-242.


Creative Commons License
Indonesian Fisheries Research Journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View My Stats
p-ISSN 0853-8980
e-ISSN 2502-6569

Find in a library with WorldCatCrossref logoSHERPA/RoMEO Logogoogle scholardoaj