PENINGKATAN KEKEBALAN SPESIFIK ANTI STREPTOCOCCUS PADA BUDI DAYA IKAN NILA

Abstract

Uji lapang vaksin Streptococcus telah dilakukan dengan tujuan untuk mengetahui efektivitas vaksin dan respon kebal pada ikan nila (Oreochromis niloticus) terhadap rangsangan yang diberikan. Penelitian dilakukan pada keramba jaring apung (KJA). Vaksin yang digunakan adalah vaksin S1N8 dan GM2.4 berupa vaksin yang diinaktivasi dengan formalin 0,3%. Aplikasi vaksin dilakukan secara bertahap yaitu vaksin awal (priming) diberikan melalui rendaman, sedangkan vaksin ulang diberikan melalui suntikan. Dosis vaksin awal yaitu 10 mL vaksin/100 L air untuk 1.000 ekor ikan direndam selama 15 menit, sedangkan booster diberikan melalui suntikan 0,2 mL/ ekor ikan. Hasil menunjukkan bahwa kombinasi pemberian vaksin priming melalui rendaman dan booster dengan suntikan untuk vaksin S1N8 menghasilkan sintasan paling tinggi (70,3%—72,5%), apabila dibandingkan dengan vaksin GM2.4 (59,3%— 62,5%) dan kontrol (35,5%—42,0%).

Field study of vaccines S1N8 and GM2.4 with the aims to evaluate the effectiveness of vaccine and the immune response of nile tilapia ( Oreochromis niloticus ) against the vaccines. The research have been conducted in floating net cage. Vaccine tested were produced from Streptococcus iniae isolates S1N8 and GM2.4 which was prepared by formalin killed of 0.3%(v/v). Vaccine delivery were given in two steps i.e. priming with immersion, and booster through injection. The dose of vaccine for priming was 10 mL of vaccine/100 L water immersed for 1,000 fish for 15 minutes. Booster were delivered by injection as much as 0.2 mL/fish. The results indicated that combination of vaccine delivering of immersion (priming) and injection (booster) especially for S1N8 vaccine were the highest percent of survival rate (70.3%—72.5%) as compared with GM2.4 vaccine (59.3%—62.5%) and control (35.5%—42.0%).