AKTIVITAS KITINASE, LESITINASE, DAN HEMOLISIN ISOLAT DARI BAKTERI IKAN NILA (Oreochromis niloticus Lin.) YANG DIKULTUR DALAM KERAMBA JARING APUNG WADUK JATILUHUR, PURWAKARTA

Wibowo Mangunwardoyo, Ratih Ismayasari, Etty Riani

Abstract


Aeromonas hydrophila Lin. merupakan bakteri patogen oportunistik akuatik yang virulensinya dipengaruhi oleh adanya enzim kitinase, lesitinase, dan toksin haemolisin, merupakan penyebab kematian ikan nila yang tinggi. Penelitian bertujuan untuk mengamati aktivitas enzim kitinase, lesitinase, dan toksin hemolisin dari 30 ikan nila dari keramba jaring apung waduk Jatiluhur dengan metode tehnik agar. A. hydrophila menunjukkan positif virulen ditunjukkan adanya zona bening untuk lesitinase sebesar 7,9 mm; kitinase 8,0 mm; dan hemolisin 6,6 mm dibandingkan dengan isolat Enterobacter sp., Pseudomonas sp., dan Vibrio sp. Hal ini menunjukkan bahwa A. hydrophila bersifat patogen dan virulen terhadap ikan nila.

Aeromonas hydrophila Lin. is one of opportunistic aquatic pathogen bacteria where its pathogenic behavior is influenced by chitinase, lechitinase, and toxin haemolycine, and causes high mortality in nile tilapia culture. The purpose of the research was to observe the activities of two A. hydrophila’s enzymes i.e.: chitinase and lechitinase, and one extracelullar toxin, haemolycine, isolated from 30 nile tilapias cultured in floating net cage at Jatiluhur using quantitative plate assay technique. A. hydrophila was positive virulent marked with transparent zone of lechitinase of 7.9 mm, haemolycin of 6.6 mm, and chitinase of 8.0 mm compared to Enterobacter sp., Pseudomonas sp., and Vibrio sp. Therefore, A. hydrophila is determined as highly pathogenic bacterium and virulent for nile tilapia.


Keywords


Aeromonas hydrophila; kitinase; lesitinase; hemolisin

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DOI: http://dx.doi.org/10.15578/jra.4.2.2009.257-265


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