THE EFFECT OF THYROXINE HORMONE AND RECOMBINANT GROWTH HORMONE (RGH) SOAKING ON THE RATE OF EGG YOLK ABSORPTION AND GROWTH OF TAWES FISH (Barbonymus gonionotus)

Tristiana Yuniarti, Sri Hastuti, Ristiawan Agung Nugroho, Fajar Basuki, Diana Chilmawati, Raka Gilang Saputra

Abstract


Tawes fish (Barbonymus gonionotus), also known as lampan, is an indigenous freshwater species with significant economic value and cultivation potential. However, suboptimal growth remains a common challenge in aquaculture. Hormonal treatments such as thyroxine and rGH are known to enhance metabolic processes, potentially improving egg yolk absorption and larval growth. This study aimed to evaluate the effects of thyroxine and rGH hormone immersion on the rate of egg yolk absorption and development of newly hatched tawes fish larvae. The experimental design employed a CRD with four treatments and three replications: A (thyroxine 0 mg/L + rGH 2.5 mg/L), B (thyroxine 0.1 mg/L + rGH 0 mg/L), C (thyroxine 0 mg/L + rGH 2.5 mg/L), and D (thyroxine 0.1 mg/L + rGH 2.5 mg/L), with hormone immersion conducted for 60 minutes. The results demonstrated that the combined immersion of thyroxine and rGH significantly (P <0.05) improved the absolute weight gain, absolute length increment, and survival rate of tawes fish larvae. The highest values for absolute weight (0.043±0.002 g) and length (12.77±0.15 mm) were observed in treatment D (thyroxine 0.1 mg/L + rGH 2.5 mg/L), while the best survival rate (70.00±2.00%) was recorded in treatment A (thyroxine 0 mg/L + rGH 2.5 mg/L). These findings suggest that the combined use of thyroxine and rGH can effectively enhance the early growth and development of tawes fish larvae.

Keywords


Tawes fish; larvae growth; Recombinant Growth Hormone (rGH); Yolk absorption rate

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References


Abdel-Tawwab, M., Mousa, M. A. A., & Sharaf, S. M. (2010). Growth performance and survival rate of Nile tilapia (Oreochromis niloticus) early fry as affected by different dietary protein and stocking density levels. Aquaculture Research, 41(5), 594–601. https://doi.org/10.1111/j.1365-2109.2009.02349.x

Alimuddin, Lesmana, I., Sudrajat, A. O., Carman, O., & Faizal, I. (2010). Production and bioactivity potential of three recombinant growth hormones of farmed fish. Indonesian Aquaculture Journal, 5(1), 11–17. https://dx.doi.org/10.15578/iaj.5.1.2010.11-17

Andriawan, R., Basuki, F., & Yuniarti, T. (2020). Effect of soaking time of thyroxine hormone (T4) on growth and survival of white tilapia larvae (Oreochromis niloticus). Tropical Aquaculture Science: Indonesian Journal of Tropical Aquaculture, 4(1), 51–60. https://doi.org/10.14710/sat.v4i1.5473

Apriliani, R. F., Basuki, F., & Nugroho, R. A. (2018). The effect of giving recombinant growth hormone (rGH) with different doses in artificial feed on the growth and survival of tawes fish seeds (Puntius sp.). Tropical Aquaculture Science: Indonesian Journal of Tropical Aquaculture, 2(1), 49-58. https://doi.org/10.14710/sat.v2i1.2561

Atmojo, A., Basuki, F., & Nugroho, R. A. (2017). The effect of giving recombinant growth hormone (rGH) through the immersion method with different lengths of time on the growth and survival of freshwater pomfret larvae (Colossoma macropomum Cuv.). Journal of Aquaculture Management and Technology, 6(3), 1–9.

Ayyub, H., Budiharjo, A., & Sugiyarto, S. (2019). Morphological characteristics of silver barb fish population Barbonymus gonionotus (Bleeker, 1849) from different water locations in Central Java Province. Jurnal Iktiologi Indonesia, 19(1), 65- 78. https://doi.org/10.32491/jii.v19i1.378

Bagenal, T. B. (1978). Methods for assessment of fish production in fresh waters (3rd ed.). Oxford: Blackwell Science.

Boyd, C. T. (1979). Water quality in warmwater fish ponds. Auburn University Press.

Brett, J. R., & Groves, T. D. D. (1979). Physiological energetics. In W. S. Hoar, D. J. Randall, & J. R. Brett (Eds.), Fish physiology: Volume VIII. Bioenergetics and growth (pp. 279–352). Academic Press.

Dedi, D. (2018). The influence of giving thyroxine hormone in Megami pellet feed on the growth of cantang grouper seeds (Epinephelus fuscoguttatus × E. lanceolatus). Intek Aquaculture, 2(2), 33–48.

Elvarianna, G., Tang, U. M., & Rusliadi. (2017). Growth and survival of mouse grouper seeds (Cromileptus altivelis) with recombinant growth hormone (rGH) administration through different dose immersion methods. Online Journal of Students of the Faculty of Fisheries and Marine Sciences, University of Riau, 4(1), 1–9.

Erlangga, E., Zulfikar, & Hariyati, H. (2017). Effect of thyroxine and recombinant growth hormone on growth and survival of goldfish larvae (Carassius auratus). Indonesian Journal of Ichthyology, 19(1), 274–339.

Hanief, M. A. R., Subandiyono, & Pinandoyo. (2014). The effect of feeding frequency on the growth and survival of tawes (Puntius javanicus) seeds. Journal of Aquaculture Management and Technology, 3(4), 67–74.

Hastuti, S., Yuniarti, T., Harwanto, D., Subandiyono, S. (2024). Growth performance of Java barb, Barbonymus gonionotus, in relation with water temperature. AACL Bioflux. 17. 949-959. http://www.bioflux.com.ro/docs/2024.949-959.pdf#

Hemming, T. A., & Buddington, R. K. (1988). Yolk absorption in embryonic and larvae fishes. In W. S. Hoar & D. J. Randall (Eds.), Fish physiology: The physiology of developing fish, egg and larvae (Vol. XI, Part A). Academic Press.

Heraedi, A., Prayitno, S. B., & Yuniarti, T. (2018). The effect of different thyroxine hormone (T4) concentration on the growth, survival, and pigment development of pink zebra fish larvae (Brachydanio rerio). Omni-Aquatics, 14(2), 21–28.

Ihsanudin, I., Rejeki, S., & Yuniarti, T. (2014). The effect of administration of recombinant growth hormone (rGH) through oral method with different time intervals on the growth and survival of Larasati tilapia seeds (Oreochromis niloticus). Journal of Aquaculture Management and Technology, 3(2), 94–102.

Karimah, U., Samidjan, I., & Pinandoyo. (2018). Growth performance and survival of GIFT tilapia (Oreochromis niloticus) given different amounts of feed. Journal of Aquaculture Management and Technology, 7(1), 128–135.

Khalil, N. A., Alya, H. M. M. K., & Mousa, M. A. (2011). The effect of maternal thyroxine injection on growth, survival, and development of the digestive system of Nile tilapia (Oreochromis niloticus) larvae. Advances in Bioscience and Biotechnology, 2, 320–329.

Kurniawan, O., Johan, T. I., & Setiaji, J. (2014). The effect of giving thyroxine hormone (T4) by soaking on the growth and survival rate of gourami fish seeds (Osphronemus gouramy). Agricultural Dynamics, 29(1), 107–112.

Moniruzzaman, M., Uddin, K. B., Basak, S., & Bashar, A. (2015). Effects of stocking density on growth performance and yield of Thai silver barb (Barbonymus gonionotus) reared in floating net cages in Kaptai Lake, Bangladesh. AACL Bioflux, 8(6), 999–108.

Mulyani, Y. W. T., Solihin, D. D., & Affandi, R. (2015). Efficiency of egg yolk absorption and morphogenesis of silver arowana prelarvae (Osteoglossum bicirrhosum) at various temperature and salinity interactions. Indonesian Journal of Ichthyology, 15(3), 179–191.

Muslim, M., Hanief, M., & Widodo, M. S. (2019). Influence of thyroxine hormone on metabolic rate and growth of tawes fish larvae. Indonesian Journal of Fisheries Research, 25(3), 213–220.

National Research Council. (2012). Nutrient requirements of swine (11th rev. ed.). The National Academies Press. https://doi.org/10.17226/13298

Nisa, A. C., & Khairunissa, A. F. (2023). The Breeding Technique of Tawes Fish (Barbonymus Gonionotus) in Regional Technical Implementation Unit (UPTD) for Freshwater Aquaculture in Jember. Chanos Chanos, 21(2), 83-89. https://dx.doi.org/10.15578/chanos.v21i2.13556

Nuswantoro, S., Pradhana, A., Kusumah, R. V., & Fariedah, F. (2019). Relationship between egg yolk absorption rate and growth of maanvis fish larvae (Pterophyllum scalare). Journal of Fisheries and Marine Research (JFMR), 3(2), 155–157.

Pasaribu, A. F., Muslim, M., & Syaifudin, M. (2019). Effect of soaking time in thyroxine solution on the growth and survival of snakehead fish larvae (Channa striata). Indonesian Swamp Aquaculture Journal, 7(1), 25–33.

Pratama, B. A., Susilowati, T., & Yuniarti, T. (2018). The Effect of Different Temperature in Eggs Hatching Time, Hatching Rate, Survival Rate and Growth of Gourami Fish (Osphronemus gouramy) strain Bastar. Jurnal Sains Akuakultur Tropis, 2(2018), 59–65. https://doi.org/10.14710/sat.v2i1.2478

Samad, A. P. A., Navia, Z. I., Junita, A., & Syahrial, S. (2025). Morphometric and meristic variations of Java Barb (Barbonymus gonionotus) in six districts of Aceh Province, Indonesia. Egyptian Journal of Aquatic Research, 51(1), 81-90.

Setiawan, B., Rahmawati, D., & Handayani, S. (2022). Effectiveness of thyroxine hormone administration on growth and nutrient absorption in fish larvae. Journal of Aquatic Biosciences, 4(1), 15–22.

Steffens, W. (1989). Principles of fish nutrition. Ellis Horwood Ltd.

Sudrajat, A. O., & Muttaqin, M. (2013). Effectiveness of thyroxine and recombinant growth hormone on the growth of Siam-catfish larvae. Indonesian Journal of Aquaculture, 12(1), 31–39.

Tamsil, A. A., & Hasanidar, H. (2024). Reproductive biology of silver barb, Barbonymus gonionotus (Bleeker, 1850), in Lake Tempe, Indonesia. Iranian Journal of Fisheries Sciences, 20(6), 1324-1336. http:doi.org/ 10.22092/ijfs.2024.131908




DOI: http://dx.doi.org/10.15578/iaj.20.1.2025.37-47

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