RESPONS PERTUMBUHAN, PEMANFAATAN NUTRIEN, DAN AKTIVITAS ANTIOKSIDAN IKAN TORSORO (Tor soro) YANG DIBERI PAKAN DENGAN SUPLEMENTASI GLUTAMIN
Abstract
Penelitian ini bertujuan untuk mengevaluasi pengaruh suplementasi glutamin terhadap pertumbuhan dan aktivitas antioksidan pada ikan Torsoro (Tor soro). Ikan uji yang digunakan adalah juvenil ikan dengan berat awal rata-rata 5,21 ± 0,04 g. Perlakuan yang diberikan terdiri atas suplementasi glutamin bebas dengan dosis yang berbeda (0; 0,5; 1,0; 1,5; dan 2%) dan alanil-glutamin (0,84; 1,67; 2,51; dan 3,35%) pada pakan, setiap perlakuan memiliki lima kali ulangan. Perlakuan pakan diberikan tiga kali sehari sekenyangnya. Ikan uji dipelihara selama 60 hari. Parameter yang diamati meliputi performa pertumbuhan, pemanfaatan nutrisi, indeks biologis, dan aktivitas antioksidan. Hasil penelitian menunjukkan bahwa pakan yang disuplementasi glutamin bebas berpengaruh nyata (p<0,05) terhadap performa pertumbuhan, pemanfaatan nutrisi, indeks biologis, dan aktivitas antioksidan ikan uji. Hasil uji lebih lanjut menunjukkan bahwa suplementasi alanil-glutamin dalam pakan sebesar 2,51% memberikan hasil terbaik terhadap bobot akhir, laju pertumbuhan spesifik, retensi protein, rasio efisiensi protein, konversi pakan, rasio viseral somatik, rasio panjang usus, aktivitas superoksida dismutase, glutation peroksidase, dan katalase pada ikan Torsoro. Berdasarkan evaluasi keseluruhan terhadap parameter yang diamati, penelitian ini menunjukkan bahwa suplementasi alanil-glutamin dalam pakan pada tingkat 2,51% mampu meningkatkan kinerja pertumbuhan, pemanfaatan nutrisi, indeks biologis, dan aktivitas antioksidan pada ikan Torsoro.
This study aimed to evaluate the effects of glutamine supplementation on the growth of and antioxidant activity in Torsoro fish (Tor soro). The test fish used were Torsoro fish juveniles with an average initial weight of 5.21 ± 0.04 g. The treatments consisted of different supplementation levels of free glutamine (0; 0.5; 1.0; 1.5; and 2%) and alanyl-glutamine (0.84; 1.67; 2.51; and 3.35%) in feed, where each treatment had five replicates. The feed treatments were given three times a day ad satiation. The experimental fish were reared for 60 days. The parameters observed included growth performance, nutrient utilization, biological indices, and antioxidant activity. The results showed that free glutamine-supplemented feed significantly affects (p<0.05) the growth performance, nutrient utilization, biological indices, and antioxidant activity of the tested fish. Further test results showed that alanyl glutamine supplementation in feed at 2.51% produced the best results on the final weight, specific growth rate, protein retention, protein efficiency ratio, feed conversion, somatic visceral ratio, intestine length ratio, superoxide dismutase, glutathione peroxidase, and catalase activities of Torsoro fish. Based on the overall achievement of the observed parameters, this study determines that alanyl-glutamine supplementation in feed at 2.51% improves the growth performance, nutrient utilization, biological indices, and antioxidant activity of Torsoro fish.
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