Efektivitas Bahan Kimia Dalam Mengobati Penyakit Motile Aeromonads Septicemia Pada Ikan Nila (Oreochromis niloticus)

Dian Eka Ramadhani, Rifqah Pratiwi, Novayanti Magdalena Gultom, Rafi Fathul Hakim, Monic Hapsari, Sofyan Alhaq, Indy Widiyanti, Khaerunisa Agustina, Abrisam Hadi Bintoro, Rega Maulana, Muhammad Erlan Hafid, Nazla Wafi Nurrafa

Abstract


Penelitian ini bertujuan untuk mendapatkan jenis dan dosis terbaik pada bahan kimia untuk mengobati penyakit motile aeromonads septicemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila pada ikan nila. Penelitian ini terdiri dari 18 perlakuan in vitro, antara lain kontrol, kalium permanganat 0,003 ppt, 0,006 ppt, 0,009 ppt; methylene blue 0,5 ppt, 1 ppt, 1,5 ppt, 2 ppt, 2,5 ppt, 3 ppt; dan garam ikan 5 ppt, 10 ppt, 15 ppt, 20 ppt, 25 ppt, 30 ppt; dengan pengujian in vitro secara tunggal, gabungan, dan bersama menggunakan metode Kirby–Bauer dan Total Plate Count (TPC), serta perlakuan in vivo pada dosis in vitro terbaik. Parameter yang diamati diantaranya zona hambat dan total bakteri patogen. Hasil penelitian menunjukkan bahwa dosis terbaik pada penelitian ini adalah perlakuan garam ikan dengan dosis 20 ppt. Hasil uji tunggal menunjukkan perlakuan garam ikan dengan dosis 20 ppt menghasilkan zona hambat terbesar 17,85 mm. Hasil pengujian gabungan antara garam ikan dan methylene blue menghasilkan zona hambat terbesar 12,1 mm dan penurunan total bakteri patogen hingga 9,1 x 108 CFU/mL. Hasil pengujian secara in vivo diperoleh hasil kelangsungan hidup ikan nila sebesar 80% pasca perlakuan garam ikan dengan dosis 20 ppt melalui metode perendaman selama 10 menit.

This research aims to obtain the best type and dose of chemicals to treat motile aeromonads septicemia (MAS) disease caused by Aeromonas hydrophila bacteria in tilapia fish. This research consisted of 18 in vitro treatments, including control, potassium permanganate 0.003 ppt, 0.006 ppt, 0.009 ppt; methylene blue 0.5 ppt, 1 ppt, 1.5 ppt, 2 ppt, 2.5 ppt, 3 ppt; and fish salt 5 ppt, 10 ppt, 15 ppt, 20 ppt, 25 ppt, 30 ppt; with single, combined, and joint in vitro testing using the Kirby–Bauer and Total Plate Count (TPC) methods, as well as in vivo treatment at the best in vitro dose. The parameters observed include the inhibition zone and total pathogenic bacteria. The research results showed that the best dose in this study was fish salt treatment with a dose of 20 ppt. The results of a single test showed that fish salt treatment with a dose of 20 ppt produced the largest inhibition zone of 17.85 mm. The results of the combined test between fish salt and methylene blue produced the largest inhibition zone of 12.1 mm and a reduction in total pathogenic bacteria of up to 9.1 x 108 CFU/mL. In vivo test results showed that tilapia survival was 80% after treatment with fish salt at a dose of 20 ppt using the soaking method for 10 minutes.


Keywords


Aeromonas hydrophila; garam ikan; kalium permanganate; methylene blue; in vitro; in vivo

Full Text:

PDF

References


Abdelhamed, A.M., El-Dee, H.A.A.E, Awad, W.H.A., & Elrefaey, N.M. (2022). Effect of using 5% potassium permanganate dressing solution on accelerating cellulitis healing process. Egyptian Journal of Health Care, 13(4), 1588-1600.

Akbar, A.Y. (2022). Pengaruh penambahan garam ikan dan probiotik terhadap kualitas air pada ikan guppy Poecilla reticulata. Panthera: Jurnal Ilmiah Pendidikan Sains dan Terapan, 2(4), 246-257.

Duncan, T.O. (1978). The use of potassium permanganate (KMnO4) in fisheries; a literature review. National Technical Information Service, PB-275-397, FWS-LR-74-14 (revised), Washington D.C.

Li, S., Cui, Y., & Wen, M.J.I.G. (2023). Toxic effects of methylene blue on the growth, reproduction, and physiology of Daphnia magna. Journal Toxics, 11(594), 1-18.

Marking, L. L., & Bills, T. D. (1975). Toxicity of potassium permanganate to fish and its effectiveness for detoxifying antimycin. Transactions of the American Fisheries Society, 104:579–583.

Miron, D. S., Silva, L. V., Golombieski, J. I., & Baldisserotto, B. (2012). Efficacy of different salt (NaCl) concentrations in treatment of Ichthyophthirius multifilis-infected silver catfish, Rhamdia quelen, fingerlings. Journal of Applied Aquaculture, 14(1-2), 155-161.

Muslikha, Pujiyanto, S., Jannah, S.N., & Novita, H. (2016). Isolasi, karakterisasi Aeromonas hydrophila dan deteksi gen penyebab penyakit Motile Aeromonas Septicemia (MAS) dengan 16s rRNA dan Aerolysin pada ikan lele Clarias sp. Jurnal Biologi, 5(4), 1-7.

Pandey, G. (2014). Potassium permanganate for the treatment of external infections of fish. Journal Jigyasa-I, 8(1), 73-75.

[PERMENKP] Peraturan Menteri Kelautan dan Perikanan Republik Indonesia. (2019). PERMENKP Nomor:1/PERMEN-KP/2019 tentang obat ikan. https://peraturan.bpk.go.id/Details/159085/permen-kkp-no-1permen-kp2019-tahun-2019. [9 Juni 2024].

Pratiwi, R. (2016). Budidaya dengan sistem kompartemen individu terhadap respons fisiologis dan kinerja produksi lobster pasir Panulirus homarus. [Tesis]. Sekolah Pascasarjana Institut Pertanian Bogor (ID).

Pratiwi, R., Hidayat, K.W., & Sumitro, S. (2020). Production performance of catfish Clarias gariepinus Burchell, 1822 cultured with added probiotic Bacillus sp. on biofloc technology. Journal of Aquaculture and Fish Health, 9(3), 274-285.

Pratiwi, R., Kusuma, N.P.D., Serihollo, L.G.G., Amalo, P., Suhono, L., & Kartika, I.W.D. (2023). Application of kajarula technology to the productivity of seaweed Kappaphycus striatus at Tablolong Beach, West Kupang, East Nusa Tenggara. E3S Web of Conferences, 442, 02032.

Pratiwi, R., Supriyono, E., & Widanarni. (2016). Total hemocytes, glucose hemolymph, and production performance of spiny lobster Panulirus homarus cultured in the individual compartments system. Jurnal Ilmu dan Teknologi Kelautan Tropis, 8(1), 321-333.

Ramadhani, D.E., Pratiwi, R., Gultom, N.M., Hakim, R.F., Hapsari, M., Alhaq, S., Maula, A., Fauziah, S.S., Hafid, M.E., & Nurrafa, N.W. (2023). Diagnosa agen penyakit ikan di Kecamatan Cibereum, Sukabumi, Jawa Barat. Jurnal Megaptera, 2(1), 15-24.

Roeswandono, Putri, A.S., Sasmita, R., & Rahmawati, I. (2021). Pengendalian infestasi ektoparasit Argulus sp. pada benih ikan mas Cyprinus carpio dengan menambahkan garam (NaCl) di Pasar Ikan Hias Gunung Sari Surabaya. Jurnal Vitek Bidang Kedokteran Hewan, 11(2), 20-25.

Sarjito, P., Slamet, B., & Haditomo, A.H.C. (2013). Buku pengantar parasit dan penyakit ikan. Fakultas Perikanan dan Ilmu Kelautan Universitas Diponegoro. Semarang. pp 14-17.

Straus, D.L., & Griffin, B.R. (2002). Efficacy of potassium permanganate in treating Ichthyophthiriasis in channel catfish. Journal of Aquatic Animal Health, 14: 145-148.

Sulistiani, Surianti, & Putri, A.R.S. (2023). Pengaruh dosis obat methylene blue terhadap kelangsungan hidup ikan nila Oreochromis niloticus yang terserang penyakit white spot. Jurnal Sains dan Teknologi Perikanan, 3(2), 77-85.

Thesnaar, L., Bezuidenhout, J.J., Petzer, A., Petzer, J.P., & Cloete, T.T. (2021). Methylene blue analogues: in vitro antimicrobial minimum inhibitory concentrations and in silico pharmacophore modelling. European Journal of Pharmaceutical Sciences, 1(1), 157.

Wardani, D.W., Purnama, A.M., Jaziri, A.A., & Kartikaningsih, H. (2021). Effect of sodium chloride concentration on Aeromonas hydrophila, proximate, and organoleptic analysis in catfish Clarias sp. Journal of Aquaculture and Fish Health, 10(2), 199.




DOI: http://dx.doi.org/10.15578/jmtr.v2i2.14350

Refbacks

  • There are currently no refbacks.


Pusat Penelitian dan Pengabdian kepada Masyarakat

Politeknik Kelautan dan Perikanan Kupang

Jl. Kampung Baru, Pelabuhan Ferry Bolok, Kecamatan Kupang Barat, Kabupaten Kupang, Nusa Tenggara Timur, 85351

P-ISSN: 29862310 

E-ISSN: 29860903 

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

View My Stats