Pengujian Obat Kimia untuk Menghambat Pertumbuhan Bakteri Patogen Aeromonas hydrophila Secara In Vitro dan In Vivo

Abstract

This study aims to obtain the best type of chemical drug and dosage to inhibit the growth of pathogenic bacteria Aeromonas hydrophila (AHA). This study was conducted at the School Health and Environment Laboratory, Fish Seed Technology and Management Study Program, IPB Vocational School, Sukabumi Campus. The experimental design carried out in this study consisted of 4 treatments, including control (K), fish salt 5 g / L, 10 g / L, 15 g / L, 20 g / L, 25 g / L, 30 g / L, methylene blue 0.5 mL / L, 1 mL / L, 1 mL / L, 1 mL / L, 1.5 mL / L, 2 mL / L, 2.5 mL / L, 3 mL / L, lukastop 0.01 mL / L, 0.013 mL / L. Drug testing was carried out in vitro with single and combined testing of each drug. In vivo testing was carried out with the best dose from the in vitro results tested on fish as a treatment for AHA bacterial infections. The observed in vitro parameters were the inhibition zone and total pathogenic bacteria Aeromonas hydrophila, while the observed in vivo parameters were Survival Rate (SR), Daily Growth Rate (LPH), and Mortality Rate (MR). The best vitro test results in a single test were obtained in the treatment of methylene blue 3 mL/L with an inhibition zone of 18.25 mm and the lowest number of AHA bacteria, namely 5.197 × 109 CFU/mL. The best in vitro test results in the combined test were obtained in the treatment of 25 g/L fish salt and 3 mL/L methylene blue with an inhibition zone of 17.9 mm. The results of vivo therapy testing with methylene blue 3 mL/L produced an SR value of 80% and were able to help post-infection healing and increase fish appetite.