NUTRITIONAL AND MICROBIOLOGICAL PROFILE OF FERMENTED SKIPJACK TUNA VISCERA SILAGE BASED ON MOLASSES VARIATIONS AS A CANDIDATE FOR VALUE-ADDED FEED INGREDIENTS

IGP Gede Rumayasa Yudana, Fitroh Fitroh Dwi Hariyoto, Hetty MAGRITTHA PAULINE Ondang, Nova MAGDALENA TUMANDUK, Itje ANTI WEWENGKANG, Fidel JAMES BONIFACIO Ticoalu, mohamad akbar, Fernando Wowiling, Dyah ayu Rakhmayeni, DESSY ATIKA NATALIA, Amelia Abdillah

Abstract


This study aims to evaluate the nutritional and microbiological profiles of fermented skipjack tuna (Katsuwonus pelamis) entrails silage based on variations in molasses addition as a candidate for value-added feed ingredients. The study was conducted from September to November 2025 at the Processing Workshop of the Bitung Marine and Fisheries Polytechnic. The skipjack tuna entrails raw material was fermented for 14 days using probiotics as a source of Lactic Acid Bacteria (LAB) with molasses added at levels of 10%, 20%, and 30%. The parameters tested included crude protein, crude fat, Total Plate Count (TPC), total LAB, and the presence of Salmonella. The results showed that increasing the molasses level tended to decrease the protein content and produced variations in crude fat values in the silage product. Microbiological activity showed that LAB was higher in the 30% molasses treatment, while TPC was relatively stable in all treatments. All samples were declared safe for because they were Salmonella-negative. Overall, fermented skipjack tuna entrails silage with a molasses level of 10% provided a more optimal nutritional and microbiological profile than other treatments, making it a potential candidate for value-added feed ingredients in supporting the sustainable utilization of fishery waste

Keywords


skipjack tuna silage; molasses; nutrition; microbiology; value-added

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References


Andriani, Y., & Aisyah, A. (2025). Making Silase of Trash Fish Using Bacteri Asam Lactate. GPH-International Journal of Agriculture and Research, 8(7), 31–41. https://doi.org/10.5281/zenodo.16941021

Bawono, W. B., Rahardja, B. S., & Prayogo, P. (2015). Substitusi Silase secara Kimiawi Limbah Padat Surimi Ikan Swanggi (Priacanthus macracanthus) pada Tepung Ikan terhadap Retensi Energi dan Rasio Konversi Pakan Benih Ikan Nila (Oreochromis niloticus). Jurnal Ilmiah Perikanan Dan Kelautan, 7(2), 177–182.

Cortés-Sánchez, A. D. J., Jaramillo-Flores, M. E., Díaz-Ramírez, M., Espinosa-Chaurand, L. D., & Torres-Ochoa, E. (2024). Biopreservation and the Safety of Fish and Fish Products, the Case of Lactic Acid Bacteria: A Basic Perspective. Fishes, 9(8), 303. https://doi.org/10.3390/fishes9080303

Fagbenro, O. A., & Jauncey, K. (1994). Chemical and nutritional quality of dried fermented fish silages and their nutritive value for tilapia (Oreochromis niloticus). Animal Feed Science and Technology, 45(2), 167–176. https://doi.org/10.1016/0377-8401(94)90024-8

Handajani, H. (2014). Peningkatan Kualitas Silase Limbah Ikan Secara Biologis Dengan Memanfaatkan Bakteri Asam Laktat. JURNAL GAMMA, 9(2), 31–39.

Jayanti, Z. D., Herpandi, H., & Lestari, S. D. (2018). Pemanfaatan Limbah Ikan Menjadi Tepung Silase dengan Penambahan Tepung Eceng Gondok (Eichhornia crassipes). FishtecH - Jurnal Teknologi Hasil Perikanan, 7(1), 86–97.

Kim, Y. J., Youk, S., & Song, C. S. (2022). Effectiveness of Administering a Mixture of Lactic Acid Bacteria to Control Salmonella ser. Enteritidis Infections in Broilers. Animals, 12(3), 374. https://doi.org/10.3390/ani12030374

La Apu, R. G. (2021). Pemanfaatan Limbah Jeroan Ikan Cakalang (Katsuwonus pelamis) sebagai Bahan Subtitusi Tepung Ikan pada Pertumbuhan Ikan Nila (Oreochromis niloticus). Jurnal Sains Dan Teknologi Perikanan, 1(2), 13–24. https://doi.org/10.55678/jikan.v1i2.528

Makodompit, A., & Minarti, M. (2025). Inovasi Pengolahan Ikan dalam Perspektif Ekonomi Biru. Imajinasi : Jurnal Ilmu Pengetahuan, Seni, Dan Teknologi, 2(2).

Maksimenko, A., Belyi, L., Podvolotskaya, A., Son, O., & Tekutyeva, L. (2024). Exploring Sustainable Aquafeed Alternatives with a Specific Focus on the Ensilaging Technology of Fish Waste. In Fermentation (Vol. 10, Issue 5). https://doi.org/10.3390/fermentation10050258

Nithya, G., & Suresh, R. (2025). Nutritive Value of Fish Waste Silage Meal. Archives of Current Research International, 25(7), 247–255. https://doi.org/10.9734/acri/2025/v25i71328

National Research Council (NRC). (2020). Nutrient Requirements of fish and shrimp. Washington, DC.

Nurhayati, T., Desniar, & Suhandana, M. (2013). Pembuatan pepton secara enzimatis menggunakan bahan baku jeroan ikan tongkol. JPHPI, 16(1).

Piasari, R., Soeprijadi, L., & Saputra, R. S. H. (2025). Penerapan Good Manufacturing Practice (Gmp) Dan Sanitation Standar Operating Procedure (Ssop) Pada Proses Pengolahan Tekwan Ikan Tenggiri (Scomberomorus guttatus).

Prihanto, A. A., Umam, N. I., & Bangun, J. D. G. (2024). Unveiling the Secrets of Indonesian fermented Fish: Characteristics of lactic acid bacteria, roles, and potential in product development. In Food Bioscience (Vol. 61). https://doi.org/10.1016/j.fbio.2024.104629

Putri, D. S., Miranti, S., Wulandari, R., Muzahar, M., Yulianto, T., Bakkara, O. R., Irawan, H., & Zubandi, Z. (2024). Evaluation of Nutritional Content of Fermented Fish Visceral Silage using Sorbic Acid and Effective Microorganism (EM4). BIO Web of Conferences, 134, 1–6. https://doi.org/10.1051/bioconf/202413406020

Safir, M., Bayan, F. F., Mangitung, S. F., Rusaini, & Rahman, S. A. (2024). Pengaruh Penambahan Silase Hasil Samping Pengolahan Ikan Patin Siam (Pangasius hypopthalmus) pada Pakan Terhadap Performa Pertumbuhan Udang Vaname (Penaeus vannamei). JAGO TOLIS : Jurnal Agrokompleks Tolis, 5(1), 33–39. https://doi.org/10.56630/jago.v5i1.718

Santana, T. M., Dantas, F. de M., Monteiro Dos Santos, D. K., Kojima, J. T., Pastrana, Y. M., De Jesus, R. S., & Gonçalves, L. U. (2023). Fish Viscera Silage: Production, Characterization, and Digestibility of Nutrients and Energy for Tambaqui Juveniles. Fishes, 8(2). https://doi.org/10.3390/fishes8020111

Serdiati, N., Safir, M., Tobigo, D. T., & Mansyur, K. (2022). Pembuatan Silase Limbah Ikan Patin Menjadi Bahan Baku Pakan Ikan. Jurnal Pengabdian Masyarakat, 5(2).

Sofia, A., Nugroho, B. E. L., Maulana, M. A., Silviawati, P. A., Ramadhan, S., & Sari, Y. (2021). Aplikasi Bioteknologi Dalam Pembuatan Silase Ikan Rucah Melalui Fermentasi Bakteri Asam Laktat. Jurnal Akuakultura Universitas Teuku Umar, 5(1), 10–16. https://doi.org/10.35308/ja.v5i1.3887

Sulistyoningsih, M. (2015). Pengaruh pemberian silase limbah ikan terhadap kadar protein daging dan lemak daging broiler sebagai upaya peningkatan kualitas pangan. Prosiding Seminar Masyarakat Biodiversitas Indonesia, 378–382. https://doi.org/10.13057/psnmbi/m010235

Suprihadi, S., Thaib, A., Nurhayati, N., & Handayani, L. (2023). Potensi limbah perikanan sebagai alternatif sumber kalsium alami: sebuah kajian. Acta Aquatica: Aquatic Sciences Journal, 10(2), 163–171. https://doi.org/10.29103/aa.v10i2.9755

Toppe, J., & Olsen, R. L. (2024). Fish silage production by fermentation - A manual on how microbial fermentation can turn fish waste into a valuable feed ingredient or fertilizer. In Fish silage production by fermentation. FAO. https://doi.org/10.4060/cd0799en

Trenggono, S. W., Meilano, I., Latief, H., Simamora, D. C., Sabina, A., Arthatiani, F. Y., & Radiarta, I. N. (2025). Developing a marine and fisheries blue economy index for Indonesia: A comprehensive assessment framework. Sustainable Futures, 10, 101440. https://doi.org/10.1016/J.SFTR.2025.101440

Vidotti, R. M., Viegas, E. M. M., & Carneiro, D. J. (2003). Amino acid composition of processed fish silage using different raw materials. Animal Feed Science and Technology, 105(1–4), 199–204. https://doi.org/10.1016/S0377-8401(03)00056-7




DOI: http://dx.doi.org/10.15578/voc_seminar.v3i1.20737

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