THE GROWTH KINETICS AND TOTAL LIPID CONTENT OF Thalassiosira sp. (BACILLARIOPHYCEAE) UNDER MIXOTROPHIC CONDITIONS

Kelee Ira Burgan Nodque, Cleresa Salido Dionela, Fredson Hervias Huervana, Rex Ferdinand Mallare Traifalgar

Abstract


Conventional microalgae culture is challenged by issues of light limitation and cell self-shading. This study aims to evaluate the impact of different cultivation modes on the growth and lipid content of Thalassiosira sp. The diatom, Thalassiosira sp., was grown in autotrophic, mixotrophic-suspended, and mixotrophic-biofilm conditions until the stationary phase was reached. After four (4) days of culture, analysis of the cell densities revealed a significant difference between groups, with cell densities of 7.3×105 cells mL-1 for control, 1.1×106 cells mL-1 for mixotrophic-suspended, and 1.9×106 cells mL-1 for mixotrophic-biofilm cultures. Both treatments are significantly higher than the control. However, mixotrophic-biofilm culture achieved the highest cell density among all cultivation modes, 161.81% higher than the control. The specific growth rate (SGR) of Thalassiosira sp. in mixotrophic-biofilm culture was highest among treatments, while the doubling time was significantly highest in the control. Moreover, mixotrophic-biofilm culture attained the highest biomass at 56 mg 100 mL-1. Thalassiosira sp. cultured under mixotrophic-biofilm also recorded the highest lipid content at 9.89%. It is both significantly higher than the control (3.06%) and the mixotrophic-suspended culture (6.15%). The cell density, algal biomass, and lipid content of Thalassiosira sp. under mixotrophic-biofilm culture highlight this culture strategy’s promising potential in improving microalgae growth and lipid content, ridding of light as an indispensable growth factor.

Keywords


biofilm; lipid content; mixotrophic growth; suspended culture; Thalassiosira

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DOI: http://dx.doi.org/10.15578/iaj.19.1.2024.1-9

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