Rudhy Gustiano, Muhammad Fariduddin Ath-Thar, Deni Radona, Sri Sundari, Irin Iriana Kusmini


Sheat catfishes is an economically important species group from open water in Indonesia. As a high demand in the local market, this species group was threatened from overfishing and habitat destruction. For its sustainability, sheat catfishes should have an attention to be conserved and domesticated. Population genetic study has been done to understand the diversity of sheat catfishes as the basic information for its aquaculture program. Objectives of the current study are to identify and characterize the phenotype and genotype of sheat catfishes population at Plaju, Mariana Ulu, Mariana Ilir and Meritai in the down stream of Musi River, South Sumatra Province. Phenotypic analysis based on truss morphometric used eight land-mark on the body of the observed specimens. Ten specimens from each site varying in size were measured with dial caliper with an accuracy of 0.01 mm. Meanwhile, the genetic analysis used PCR-RAPD method using OPA 06, OPA 08, and OPA 12 primers. DNA was extracted from the fin of the observed specimens. Discriminant analysis reflecting in the canonical function of truss morphometric data showed that six morphometric characters (A1, A2, A5, B2, B4, and C4) enabled to separate sheat catfishes population. The population of Mariana Ulu and Mariana Ilir spread on negative of Y axis, separately from population of Plaju and Meritai. The population of Meritai is on negative of the X axis. Genetic analyses found that the population of Mariana Ilir and Meritai have a percentage polymorphism of 22.00% which was higher than the population from Mariana Ulu and Plaju with a percentage of 18%. The population of Mariana Ilir has the highest heterozygosis value of 0.093. Kinship analysis showed that the population from Plaju and Mariana Ilir has the closest distance (0.3463) while the population of Meritai and Plaju has the farthest distance (0.5429).


Siluridae; sheat catfish; morphometric; genetic; RAPD; Musi River

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Allendorf, F.W., & Utter, F.M. (1979). Population genetics. in: fish physiology 8: bioenergetics and growth. WS Hoar, DJ Randall and JR Brett (Eds), 407–454. Academic Press, New York.

Berra, T.M. (2007). Freshwater fish distribution (p. 606). University of Chicago Press, USA.

Elvyra, R., Dedy, D. S., Ridwan, A., & Zairin, J. (2010). Study of reproductive aspects on lais fish Ompok hypophthalmus in The Kampar River, Langgam District, Pelalawan Regency, Riau Province. Jurnal Natur Indonesia, 12 (2): 117-123.

Elvyra, R. (2009). The study on genetic diversity and reproduction biology of lais fish in Kampar River, Riau. PhD Thesis. Bogor: Sekolah Pascasarjana, Institut Pertanian Bogor. 126 pp.

Eschmeyer, W.N., Ferraris, C.J., Hoang, M.D., & Long, D.J. (1998). Part II. Genera of fishes. In: Catalog of fishes fishes San Fransisco, California Academy of Science, USA. 1821-2905 p.

Gjedrem, T. (2005). Selection and breeding program in aquaculture (p. 364). Springer, Netherlands.

Gustiano, R., Oktaviani, T., Soelistyowati, D.T., Kusmini, I., Wahyutomo., & Huwoyon, G. (2013). Analysis of genotype variation and truss morphometric of three population of snakehead fish (Channa striata). Berita Biologi, 12 (3): 325-333.

Gustiano, R., Kontara, E.K., Wahyuningsih, H., Subagja, J., Asih, S., & Saputra, A. (2013). Domestication mahseer (Tor soro) in Indonesia. Communication in Agricultural and Applied Biological Science, 78 (4): 165-168.

Gustiano, R. (2003). Taxonomy and phylogeny of Pangasiidae catfishes from Asia (Ostariophysi, Siluriformes). PhD Thesis. Belgium: Katholieke Universiteit Leuven. 298 pp.

Hallerman, E. (2003). Population genetics: Principles and application for fisheries scientist (p. 475). American fisheries Society, USA.

Handayani, T., Buchar, T., & Anang, N. (2009). Aspek biologi ikan lais Sheat Fish (Siluridae) di Danau Batu dan Danau Tehang. Journal of Tropical Fisheries, 3 (2): 35 - 46.

Iskandariah., Soelistyowati, D.T., Gustiano, R., Kusmini, I.I., & Huwoyon, G.H. (2015). Genetic diversity of three populations of snakeskin gourami (Trichopodus pectoralis Regan; Osphronemidae) from Kalimantan based on RAPD analysis and truss morphometric measurement. Berita Biologi, 14 (1): 57-68.

Jusmaldi., Solihin, D.D., Affandi, R., Rahardjo, M.F., & Gustiano, R. (2017). Gonad maturity and spawning type of silurid catfishes, Ompok miostoma (Vaillant, 1902) from Mahakam watershed, East Kalimantan. Jurnal Iktiologi Indonesia, 17 (2): 201-213.

Jusmaldi., Solihin, D.D., Affandi, R., Rahardjo, M.F., & Gustiano, R. (2018). Sebaran dan kekayaan ikan lais (Famili Siluridae) di Sungai Mahakam, Kalimantan Timur. Proceeding of Biology Education, 2 (1): 18-25.

Kapuscinski, A.R., & Miller, L.M. (2007). Genetic guideline for fishery managers (p. 113). University of Minnesota, Researh report, USA.

Kusmini, I.I., Gustiano, R., & Mulyasari. (2011). Genetic characterization of kelabau fish (Osteochilus kelabau) from several locations in West Kalimantan using RAPD (Random Amplified Polymorphism DNA) methods. Berita Biologi, 10 (4), 449-454.

Kusmini, I.I., Radona, D., Ath-Thar, M.H.F., Putri, F.P., Kristanto, A.H., & Gustiano, R. (2019). Phenotypic diversity in three generations of domesticated Asian redtail catfish, Hemibagrus nemurus (Valenciennes, 1840) in Indonesia. AACL Bioflux, 12 (1): 42-50.

Lorenzen, K., Beveridge, M.C.M., & Mangel, M. (2012). Cultured fish: integrative biology and management of domestication and interactions with wild fish. Biology Review, 87, 639-660.

Minggawati, I. (2010). Food habits and availability food fish lais aborted (Ompok hypopthalmus) in the kitchen Lake City Palangkaraya. Journal of Science, 2 (2): 185-191.

Minggawati, I., Sukoso,. Bijaksana, U., & Hakim, L. (2015). Gonad maturity level of catfish Ompok hypopthalmus caught in a flooding swamp area of Rungan river Central Kalimantan related to water depth. Global Journal of fisheries and Aquaculture, 3 (2): 205-210.

Nei, M., & Tajima, F. (1981). DNA Polymorphism detectable by resctriction endonuclease. Genetics, 97, 146-163.

Nelson, S.J. (2016). Fishes of the world (p. 601). John Wiley and Son, USA.

Prasetyo D. (2005). Feeding habit and spawning season of lais (Criptoterus sp.) in fisheries reserve Sungai Sambujur, Hulu Sungai Utara Regency, South Kalimantan. Journal Fisheries Science, 7 (1): 121-127.

Pulungan, C.P., Ahmad, M., Siregar, Y.I., Ma’amoen, A., & Alawi, H. (1985). Morfometrik ikan selais Siluroidea dari perairan Kecamatan Kampar Kiri Kabupaten Kampar Riau. Pekanbaru, Pusat Penelitian Universitas Riau.

PSWG [Palembang Sanitation Working Group]. (2010). Buku putih sanitasi Kota Palembang. Program percepatan pembangunan sanitasi permukiman (PPSP - 2010). Kota Palembang. 48 p.

Radona, D., Soelistyowati, D.T., Carman, O., & Gustiano, T. (2016). Genotype diversity and morphometric of tinfoil barb Barbonymus schwanenfeldii (Bleeker 1854) from Sumatra, Java dan Kalimantan. Jurnal Iktiologi Indonesia, 16 (3), 259-268.

Rahman, A. (2010). Variation of morphological structure and Cytochrome b DNA Mitochondria of Kryptopterus spp and Ompok spp (SILURIDAE) of Batang Hari watersheds, Jambi. Thesis. Bogor: Sekolah Pascasarjana, Instutut Pertanian Bogor. 51 p.

Ryman, N., & Utter, F. (1987). Population genetics and fishery management. University of Washington, Seattle, USA.

Sjafei, D.S., Simanjuntak, C.P.H., & Rahardjo, M.F. (2008). Development of gonad maturity and spawning pattern of Ompok hypophtahlmus in floodplain of Kampar Kiri River, Riau. Jurnal Ikhtiologi Indonesia, 8(2): 93-99.

Strauss, R.E., & Bookstein, F.L. (1982). The truss: body form reconstruction in morphometries. System Zoology, 31 (2): 113-135.

Tave, D. (1993). Fish genetics for fishery managers (p. 432). Kluwer Academia Publication, Netherland.

Utomo, A.D., Adjie, S., & Asyari. (1990). Aspek biologi ikan lais di perairan Lubuk Lampam Sumatra Selatan. Buletin Perikanan Darat, 2:105-111.

Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M., & Zabeau, M. (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23 (21), 4407-4414.


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