EFFECTS OF DIETARY RECOMBINANT GROWTH HORMONE WITH WATERBORNE CALCIUM CARBONATE SUPPLEMENTATION ON GROWTH PERFORMANCE AND GH–IGF AXIS-RELATED GENE EXPRESSION IN JUVENILE SNAKEHEAD (Channa striata)

Indah Permata Sari, Alimuddin Alimuddin, Agus Oman Sudrajat, Tatag Budiardi

Abstract


This study evaluated the effects of dietary recombinant growth hormone (rGH) and waterborne calcium carbonate (CaCO3) supplementation on growth performance, feed utilization, body composition, and growth-related gene expression in snakehead (Channa striata) juveniles. The experiment was conducted for 90 days using four treatments: control (R0C0, without rGH and CaCO3 supplementation), treatment 1 (R5C0, 5 mg kg-1 rGH and without CaCO3  supplementation), treatment 2 (R5C25, rGH and 25 mg L-1 CaCO3 supplementation), and treatment 3 (R5C50 rGH and 50 mg L-1 CaCO3  supplementation), each with six replicates. The results showed that rGH significantly increased final body weight (BWt) and specific growth rate, and reduced feed conversion ratio (FCR) compared with the control. Final body weight was significantly higher in R5C0 and R5C50 than in the control, with no significant difference between these treatments. In contrast, body length increased significantly with increasing CaCO3  dose, with the highest value observed in R5C50. Feed conversion ratio decreased significantly in all rGH treatments but did not differ among R5C0, R5C25, and R5C50. Protein and lipid retention were highest in R5C0 and decreased with CaCO3  supplementation. Body calcium content increased with CaCO3  supplementation, with the highest value observed in R5C50. Gene expression analysis showed that rGH significantly increased GH and IGF-1 expression compared to the control, while no consistent significant differences were observed among combined treatments. These findings suggest that rGH predominantly enhanced weight gain and feed utilization, whereas CaCO3  supplementation under rGH treatment was associated with increased body length. The combined treatments resulted in complementary but parameter-specific effects.

Keywords


Calcium; Growth; Recombinant Growth Hormone; Channa striata

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References


Association of Official Analytical Chemists (AOAC). (1995). Official methods of analysis (16th ed.). Arlington (VA): AOAC International.

Björnsson, B. T., Stefansson, G. V., Berge, A. I., Hansen, T., & Stefansson, S. O. (1998). Circulating growth hormone levels in Atlantic salmon smolts following seawater transfer: effects of photoperiod regime, salinity, duration of exposure and season. Aquaculture. 168(1–4),121–137. doi:10.1016/s0044-8486(98)00344-5.

Cavalcante, D. D., Poliato, A. S., Ribeiro, D. C., Magalhães, F. B., & Vinícius do Carmo e Sá M. (2009). Effects if CaCO3 liming on water quality and growth performance of fingerlings of nile tilapia, Oreochromis niloticus. Acta Scientiarum. Animal Sciences. 33(3): 327-333.0. doi:10.4025/actascianimsci.v3li3.6263.

Dewi, L. K., Supriadi., & Aminah, S. (2021). Analysis of calcium (ca) levels in milkfish’s (Chanos chanos) bone using Atomic Absorption Spectrophotometry (AAS). Jurnal Akademika Kimia. 10(1), 15-19. doi: 10.22487/j24775185.2021.v10.i1.pp15-19.

Fisheries and Aquaculture Organization (FAO). (2010). Channa striata (Bloch 1973). [accessed on 24 Nov 2025]. https://www.fao.org/fishery/en/aqspecies/ 3062/en.

Fontagné, S., Silva, N., Bazin, D., Ramos, A., Aguirre, P., Surget, A., Abrantes, A., Kaushik, S. J., & Power, D. M. (2009). Effects of dietary phosphorus and calcium levels on growth and skeletal development in rainbow trout (Oncorhynchus mykiss) fry. Aquaculture. 297(1-4),141-150. doi:10.1016/j.aquaculture.2009. 09.022.

Fuentes, E., Valdés, J., Molina, A., & Björnsson, B. (2013). Regulation of skeletal muscle growth in fish by the growth hormone—insulin-like growth factor system. General and Comparative Endocrinology. 192,136-148. doi:10.1016/j.ygcen. 2013.06.009.

Gustiano, R., Kusmini, I. I., & Aththar, M. H. F. (2019). Diversity, reproductive biology, and broodstock management of snakehead (Channa striata). Bogor (IDN): IPB Press. (In Indonesian).

Halmos, G., Szabo, Z., Dobos, N., Juhasz, E., & V.Schalley, A. (2025). Growth hormone-releasing hormone receptor (GHRH-R) and its signaling. Reviews in Endocrine and Metabolic Disorder. 26(3), 343-352. doi:10.1007/s11154-025-09952-x.

Idrus, I., Wahab, S., Mustapa, F., & Wibowo, D. (2020). Comparative analysis of calcium content in milkfish by varying of wet and dry conditions using AAS instrumentation. Journal of Applied Food Technology. 7(2), 41-45. doi:10.17728/jaft.8455.

Kling, P., Jönsson, E., Nilsen, T., Einarsdóttir, I., Rønnestad, I., Stefansson, S., & Björnsson, B. (2012). The role of growth hormone in growth, lipid homeostasis, energy utilization and partitioning in rainbow trout: interactions with leptin, ghrelin and insulin-like growth factor I. General and comparative endocrinology.175(1), 153-62. doi:10.1016/j.ygcen.2011.10.014.

Lall, S., & Kaushik, S. (2021). Nutrition and metabolism of minerals in fish. Animals. 11(12),1-41. doi:10.3390/ani11092711.

Lall, S. P. (2002). Fish Nutrition, Third Edition. New York (NY): Elsevier Science.

Liao, B., Deng, A., Chen, S., Chou, M., & Hwang, P. (2007). Expression and water calcium dependence of calcium transporter isoforms in zebrafish gill mitochondrion-rich cells. BMC Genomics. 8,1-13. doi:10.1186/1471-2164-8-354.

Lin, C., & Hwang, P. (2016). The control of calcium metabolism in zebrafish (Danio rerio). International Journal of Molecular Sciences. 17(11),1-156. doi:10.3390/ijms17111783.

Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25, 402-408. doi:10.1006/meth.2001.1262.

Meidiana, A., Prayogo, & Rahardja, B. S. (2022). The effect of different stocking densities on ammonia (NH3) and nitrate (NO3) concentration on striped snakehead (Channa striata) culture in the bucket. IOP Conference Series: Earth and Environmental Science. 1036, 1-6. doi:10.1088/1755-1315/1036/1/012109.

Møller, N., & Jørgensen, J. (2009). Effects of growth hormone on glucose, lipid, and protein metabolism in human subjects.. Endocrine reviews, 30 2, 152-77. doi:10.1210/er.2008-0027.

Murshed, M. (2018). Mechanism of bone mineralization. Cold Spring Harbor Perspectives in Medicine. 8(12), 1-12. doi:10.1101/cshperspect.a031229.

Muslim, M., Iskandar, A., Hendriana, A., & Lutfi, L. (2021). Growth performance of prospective broodstock of snakehead (Channa striata) fed with Nile tilapia (Oreochromis sp.) juveniles. Jurnal Sains Terapan. 11(1),1-8. doi:10.29244/jstsv.11.1.1-8. (In Indonesian).

Mustafa, A., Widodo, M. A., & Kristianto, Y. (2012). Albumin and zinc content of snakehead fish (Channa striata) extract and its role in health. International Journal of Science and Technology. 1(2), 1-8.

Pérez-Sánchez, J., Simó-Mirabet, P., Naya-Català, F., Martos-Sitcha, J.A., Perera, E., Bermejo-Nogales, A., Benedito-Palos, L., & Calduch-Giner, J. A. (2018). Somatotropic axis regulation unravels the differential effects of nutritional and environmental factors in growth performance of marine farmed fishes. Frontiers in Endocrinology. 9687),1-24. doi: 10.3389/fendo.2018.00687.

Puspaningsih, D., Supriyono, E., Nirmala, K., Rusmana, I., Kusmana, C., & Widiyati, A. (2018). the dynamics of water quality during culture of snakehead fish (Channa striata) in the aquarium. Omni-Akuatika. 14(2),123-131. doi:10.20884/1.oa.2018.14.2.568

Reinecke, M., Björnsson, B. T., Dickhoff, W. W., McCormick, S. D., Navarro, I., Power, D. M., & Gutiérrez, J. (2005). Growth hormone and insulin-like growth factors in fish: where we are and where to go. General and Comparative Endocrinology. 142, 20–4. doi: 10.1016/j.ygcen.2005.01.016.

Sakai, H., Asami, M., Naito, H., Kitora, S., Suzuki, Y., Miyauchi, Y., Tachinooka, R., ... Kamei, J. (2021). Exogenous insulin-like growth factor 1 attenuates cisplatin-induced muscle atrophy in mice. Journal of Cachexia, Sarcopenia and Muscle. 12(6),1570–1581. doi:10.1002/jcsm.12760.

Saputra, A., Syamsunarno, M. B., & Sunarno, M. T. D. (2021). Development of seed mass production of snakehead (Channa striata) in Indonesia. IOP Publishing. 715,1-7. doi:10.1088/1755-1315/715/1/012060.

Sulistiyanto, Y., Jaya, A., Zubaidah, S., Sustiyah, S., & Jovani-Sancho, J. (2024). Nutrient content of water at different land use in peatland in Central Kalimantan, Indonesia. IOP Conf. Series: Earth and Environmental Science. 1421,1-7. doi:10.1088/1755-1315/1421/1/012002.

Taruna, Y., Salampak, S., Yulianti, N., Yupi, H., Sustiyah, S., & Indrajaya, F. (2021). Effects of groundwater application on the chemical properties of peat soil and peat water in Central Kalimantan. Soilrens. 19(1), 58-68. doi:10.24198/soilrens.v19i1.35092. (In Indonesian).

Yang, S., Zhao, L., Yan, Z., Zhongmeng, Z., Zhang, C., Gong, Q., Du, X., ... Huang, X. (2021). Improvement of skeletal muscle growth by GH/IGF growth-axis contributes to growth performance in commercial fleshy sturgeon. Aquaculture. 543(4),1-11. doi:10.1016/j.aquaculture.2021.736929.

Yoshida, T., & Delafontaine, P. (2020). Mechanisms of IGF-1-mediated signaling. Cells. 9(9),1-25. doi:10.3390/cells9091970.




DOI: http://dx.doi.org/10.15578/iaj.21.1.2026.23-33

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